文献类型：期刊文献

英文题名：Mechanism of 2,3-butanediol stereoisomers formation in a newly isolated Serratia sp T241

作者：Zhang, Liaoyuan[1];Guo, Zewang[1];Chen, Jiebo[1];Xu, Quanming[1];Lin, Hui[1];Hu, Kaihui[1];Guan, Xiong[1];Shen, Yaling[2]

机构：[1]Fujian Agr & Forestry Univ, Coll Life Sci, Key Lab Biopesticide & Chem Biol, Minist Educ, Fuzhou 350002, Fujian Province, Peoples R China;[2]E China Univ Sci & Technol, New World Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2016

卷号：6

外文期刊名：SCIENTIFIC REPORTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000368108600001)】；

基金：This work was supported by the National Natural Science Foundation of China (No. 31301858), Research Fund for the Doctoral Program of Higher Education of China (No. 20133515120011), the Outstanding Youth Fund of the Provincial Education Department of Fujian Province of China (No. JA14100), the Outstanding Youth Fund of Fujian Agriculture and Forestry University (No. xjq201408).

语种：英文

摘要：Serratia sp. T241, a newly isolated xylose-utilizing strain, produced three 2,3-butanediol (2,3-BD) stereoisomers. In this study, three 2,3-butanediol dehydrogenases (BDH1-3) and one glycerol dehydrogenase (GDH) involved in 2,3-BD isomers formation by Serratia sp. T241 were identified. In vitro conversion showed BDH1 and BDH2 could catalyzed (3S)-acetoin and (3R)-acetoin into (2S, 3S)-2,3-BD and meso-2,3-BD, while BDH3 and GDH exhibited the activities from (3S)-acetoin and (3R)-acetoin to meso-2,3-BD and (2R, 3R)-2,3-BD. Four encoding genes were assembled into E. coli with budA (acetolactate decarboxylase) and budB (acetolactate synthase), responsible for converting pyruvate into acetoin. E. coli expressing budAB-bdh1/2 produced meso-2,3-BD and (2S, 3S)-2,3-BD. Correspondingly, (2R, 3R)-2,3-BD and meso-2,3-BD were obtained by E. coli expressing budAB-bdh3/gdh. These results suggested four enzymes might contribute to 2,3-BD isomers formation. Mutants of four genes were developed in Serratia sp. T241. triangle bdh1 led to reduced concentration of meso-2,3-BD and (2S, 3S)-2,3-BD by 97.7% and 87.9%. (2R, 3R)-2,3-BD with a loss of 73.3% was produced triangle bdh3. Enzyme activity assays showed the decrease of 98.4% and 22.4% triangle bdh1 and triangle bdh3 compared with the wild strain. It suggested BDH1 and BDH3 played important roles in 2,3-BD formation, BDH2 and GDH have small effects on 2,3-BD production by Serratia sp. T241.