文献类型：期刊文献

英文题名：An efficient shortened genetic transformation strategy for filamentous fungus Trichoderma reesei

作者：Wu, Chuan[1];Chen, Yumeng[1];Huang, Xiaoxue[1];Sun, Shishuai[1];Luo, Jinnan[1];Lu, Zhiwen[1];Wang, Wei[1];Ma, Yushu[1]

机构：[1]East China Univ Sci & Technol, New World Inst Biotechnol, State Key Lab Bioreactor Engn, POB 311,130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2019

卷号：65

期号：6

起止页码：301

外文期刊名：JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000510627500005)】；

基金：This research was supported by the Open Funding Project of the State Key Laboratory of Bioreactor Engineering and College Students Innovation and Entrepreneurship Training Program of East China University of Science and Technology.

语种：英文

外文关键词：G418; optimized codons; Trichoderma reesei; two-Agrobacterium-mediated transformation

摘要：The filamentous fungus Trichoderma reesei is one of the most important fungi for the production of cellulases and xylanases, which can be used for biofuel production from lignocellulose. We aimed to develop an effective selection marker system for more extensive functional genomic studies in the fungus T. reesei, and to construct better industrial transformants for producing cellulases. Here, we present a novel effective G418 selection marker to u se a codon-optimized neomycin phosphotransferase II gene nptII to transform T. reesei. We developed an effective and erasable selection marker, IcNG, and a combined genetic transformation system for gene manipulation in T. reesei using a two-Agrobacterium-mediated transformation method. This transformation strategy combines two steps in the transformation protocol, which saves 15-30-day's time. The system could be a useful tool for the genetic engineering of T. reesei.