文献类型：期刊文献

英文题名：CqToll participates in antiviral response against white spot syndrome virus via induction of anti-lipopolysaccharide factor in red claw crayfish Cherax quadricarinatus

作者：Li, Yan-yao[1,2];Chen, Xiao-xiao[1,2];Lin, Feng-yu[1];Chen, Qiu-fan[1];Ma, Xing-yuan[2];Liu, Hai-peng[1]

机构：[1]Xiamen Univ, State Key Lab Marine Environm Sci, Fujian Collaborat Innovat Ctr Exploitat & Utiliza, Fujian Engn Lab Marine Bioprod & Technol, Xiamen 361102, Fujian, Peoples R China;[2]East China Univ Sci & Technol, Sch Biotechnol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China

年份：2017

卷号：74

起止页码：217

外文期刊名：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000403512600023)】；

基金：This study was supported by the National Natural Science Foundation of China (nos. U1605214, 41476117, 41676135), FRFCU (20720150155, 20720170083, 201610384127), the Science and technology innovation action plan of Shanghai (14431904300, 17431904600) and partially supported by Open Funding Project of the State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology.

语种：英文

外文关键词：Toll receptor; Cherax quadricarinatus; White spot syndrome virus; Anti-lipopolysacchride factor

摘要：It is well known that Tolls/Toll like receptors (TLRs), a family of pattern recognition receptors, play important roles in immune responses. Previously, we found that a Toll transcript was increased in a transcriptome library of haematopoietic tissue (Hpt) cells from the red claw crayfish Cherax quadricarinatus post white spot syndrome virus infection. In the present study, a full-length cDNA sequence of Toll receptor (named as CqToll) was identified with 3482 bp which contained an open reading frame of 3021 bp encoding 1006 amino acids. The predicted structure of CqToll protein was composed of three domains, including an extracellular domain of 19 leucine-rich repeats residues, a transmembrane domain and an intracellular domain of 138 amino acids. Tissue distribution analysis revealed that CqToll was expressed widely in various tissues determined from red claw crayfish with highest expression in haemocyte but lowest expression in eyestalk. Importantly, significant lower expression of the antilipopolysacchride factor (CqALF), an antiviral antimicrobial peptide (AMP) in crustaceans, but not CqCrustin was observed after gene silencing of CqToll in crayfish Hpt cell cultures, indicating that the CqALF was likely to be positively regulated via Toll pathway in red claw crayfish. Furthermore, the transcription of both an immediate early gene and a late envelope protein gene VP28 of WSSV were clearly enhanced in Hpt cells if silenced with CqToll, suggesting that the increase of WSSV replication was likely to be caused by the lower expression of the CqALF resulted from the loss-of-function of CqToll. Taken together, these data implied that CqToll might play a key role in anti-WSSV response via induction of CqALF in a crustacean C quadricarinatus. (C) 2017 Published by Elsevier Ltd.