文献类型：期刊文献

英文题名：Coevolution between marine Aeromonas and phages reveals temporal trade-off patterns of phage resistance and host population fitness

作者：Xu, Zhenhe[1];Ding, Zihan[1];Shi, Lijia[1];Xie, Yuzhen[1];Zhang, Yuanxing[2,3];Wang, Zhuang[1,3];Liu, Qin[1,3]

机构：[1]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai 519000, Peoples R China;[3]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai 200237, Peoples R China

年份：2023

卷号：17

期号：12

起止页码：2200

外文期刊名：ISME JOURNAL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001083282900001)】；

基金：This work was supported by the National Natural Science Foundation of China (No. 32025038), the Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism grant 2021 Sci & Tech 03-28 (Shanghai Municipal Education Commission), China Agriculture Research System of MOF and MARA (CARS-47), and ECUST-OPM Innovation Institute Open Fund (20230801).

语种：英文

摘要：Coevolution of bacteria and phages is an important host and parasite dynamic in marine ecosystems, contributing to the understanding of bacterial community diversity. On the time scale, questions remain concerning what is the difference between phage resistance patterns in marine bacteria and how advantageous mutations gradually accumulate during coevolution. In this study, marine Aeromonas was co-cultured with its phage for 180 days and their genetic and phenotypic dynamics were measured every 30 days. We identified 11 phage resistance genes and classified them into three categories: lipopolysaccharide (LPS), outer membrane protein (OMP), and two-component system (TCS). LPS shortening and OMP mutations are two distinct modes of complete phage resistance, while TCS mutants mediate incomplete resistance by repressing the transcription of phage genes. The co-mutation of LPS and OMP was a major mode for bacterial resistance at a low cost. The mutations led to significant reductions in the growth and virulence of bacterial populations during the first 60 days of coevolution, with subsequent leveling off. Our findings reveal the marine bacterial community dynamics and evolutionary trade-offs of phage resistance during coevolution, thus granting further understanding of the interaction of marine microbes.