文献类型：期刊文献

中文题名：泰乐菌素高产菌株的推理选育

英文题名：Reasonable selection of high-yielding strains of Tylosin

作者：徐红梅[1];郑黎[1];薛娟[2];陈双[2];叶蕊芳[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237;[2]河南天方药业股份有限公司,河南驻马店463003

年份：2014

卷号：46

期号：6

起止页码：12

中文期刊名：畜牧与兽医

外文期刊名：Animal Husbandry & Veterinary Medicine

收录：CSTPCD;;北大核心:【北大核心2011】；

语种：中文

中文关键词：弗氏链霉菌;泰乐菌素;诱变筛选;豆油

外文关键词：Streptomyces fradiae ; tylosin ; mutation screening ; soybean oil

摘要：以弗氏链霉菌CK6-6为出发菌株,分别采用紫外(UV)、甲基磺酸乙酯(EMS)、紫外与氯化锂(LiCl)复合、甲基磺酸乙酯与紫外复合4种方法对其进行诱变。比较了4种方法的最大提高率、致死率、正变率及突变株的传代稳定性,结果发现诱变效果较好的为EMS与UV复合诱变,筛选出1株传代稳定菌株EU45-15,相对于出发菌株CK6-6,泰乐菌素产量的提高率为15.36%。经HPLC分析发现,它的最高活性组分A的含量高于对照,提高率为48.28%。同时利用豆油作为筛选物质对弗氏链霉菌进行定向筛选,筛选效果较好,筛出1株高产稳定菌株O3.6-8,效价提高率为12.15%。
Ultraviolet （UV）, ethyl methane snlphonate （EMS）, UV and lithium chloride （LiCl） compound, EMS and UV compound were used for mutation screening on CK6-6. By comparing the maximum increase rate, the mortality rate, positive rate and the stability of mutants, we found that EMS and UV compound irradiations displayed better mutation effect on Streptomyces fradiae CK6-6. By shake flask screening, one stable high-yielding mutants was obtained： EU45-15. Tylosin yields was increased by 15.36%. HPLC analysis showed that the percentage of the highest active component A of EU45-15 was 48. 28%, higher than that of control. Simultaneously, soybean oil was used to screen high-yielding strain of S.fradiae and proved to have good effect. In the selected mutant 03.6-8, the production of tylosin was increased by 12. 15%.