文献类型：期刊文献

英文题名：Modified ferric hydroxamate spectrophotometry for assaying glycolic acid from the hydrolysis of glycolonitrile by Rhodococcus sp. CCZU10-1

作者：He, Yu-Cai[1,3]; Liu, You-Yan[2]; Ma, Cui-Luan[1,4]; Xu, Jian-He[3]

机构：[1] Laboratory of Biochemical Engineering, College of Pharmaceutical and Life Sciences, Changzhou University, Changzhou 213-164, China; [2] College of Chemistry and Chemical Engineering, Guangxi University, Nanning 530-004, China; [3] Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200-237, China; [4] Changzhou Kangpu Pharmaceutical Co., Ltd., Changzhou 213-172, China

年份：2011

卷号：16

期号：5

起止页码：901

外文期刊名：Biotechnology and Bioprocess Engineering

收录：EI(收录号：20114714536817)

语种：英文

外文关键词：High performance liquid chromatography - Hydrolysis

摘要：We successfully modified a ferric hydroxamate spectrophotometry method for assaying glycolic acid. Comparable to the high-performance liquid chromatography (HPLC)-based method, ferric hydroxamate spectrophotometry can be used to accurately monitor the time course of glycolonitrile bioconversion. Glycolic acid was assayed simply and rapidly at room temperature (25 ～ 35°C). Optimum culture conditions were obtained using this method to assay the glycolonitrile-hydrolyzing activity of Rhodococcus sp. CCZU10-1. The preferred carbon and nitrogen sources and ideal inducer were glucose (10 g/L), a composite of peptone (10 g/L) plus yeast extract (5 g/L), and e-caprolactam (2 mmol/L), respectively. The optimal growth temperature and initial medium pH for Rhodococcus sp. CCZU10-1 glycolonitrile-hydrolyzing activity were 30oC and pH 7.0. Modified ferric hydroxamate spectrophotometry could potentially be employed to assay other carboxylic acids. ? The Korean Society for Biotechnology and Bioengineering and Springer 2011.