文献类型：期刊文献

英文题名：Polymeric assembled nanoparticles through kinetic stabilization by confined impingement jets dilution mixer for fluorescence switching imaging

作者：Liu, Jingran[1];Wu, Yue[1];Tang, Jie[1];Wang, Tao[1];Ni, Feng[2];Wu, Qiumin[1];Yang, Xijiao[1];Ahmad, Ayyaz[3];Ramzan, Naveed[4];Xu, Yisheng[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Chem Engn, Shanghai 200237, Peoples R China;[2]Shanghai Shyndec Pharmaceut Co Ltd, Shanghai 200040, Peoples R China;[3]Muhammad Nawaz Sharif Univ Engn & Technol, Dept Chem Engn, Multan, Pakistan;[4]Univ Engn & Technol, Fac Chem Met & Polymer Engn, Lahore, Pakistan

年份：2023

卷号：56

起止页码：89

外文期刊名：CHINESE JOURNAL OF CHEMICAL ENGINEERING

收录：;EI(收录号：20231213745702);WOS:【SCI-EXPANDED(收录号:WOS:000957491400001)】；

基金：This work was financially supported by the National Key Research and Development Program of the International Scientific and Technological Innovation Cooperation Project among Governments (2021YFE0100400) , science and technology innovation action plan of Shanghai (22501100500) , and the International One Belt One Road Collaboration Project of Shanghai (18490740300) . The authors greatly thank Prof. A. Kayitmazer Basak at the Bogazici University for providing helpful discussions in the preparation process of NPs.

语种：英文

外文关键词：Preparation; Kinetic stabilization; Flash nanoprecipitation; Nanoparticles; Fluorescence switch

摘要：Traditional fluorescence switching molecules achieving the state change between on and off states com-monly based on UV irradiation. However, it is worth noting that UV irradiation is harmful to both the can-cer cells and the normal cells. To achieve fluorescence switching under visible wavelength and avoid complicate molecular design, a fluorophore of 2,4,5,6-tetrakis(carbazol-9-yl)-1,3-dicyanobenzene (4CzIPN) and a quencher of diarylethene (DAE) were physically incorporated within the biocompatible block copolymer poly(lactic-co-glycolic acid)-b-poly(ethylene glycol) (PLGA-b-PEG) to form 4CzIPN-DAE nanoparticles (NPs) through flash nanoprecipitation (FNP). By using the FNP method, the NPs were prepared within milliseconds in a confined impingement jets dilution (CIJ-D) mixer. Quenching and recovery of fluorescence could achieve in the presence of DAE under 475 nm and 560 nm irradiation. Appropriate structure and fluorescent properties of the nanoparticles can be tuned by external conditions for their efficient fluorescence resonance energy transfer (FRET) in a kinetic stabilization process. This NPs formation process was further optimized by varying the dilution ratio, Reynolds number (Re) and polymer concentration to modulate the mixing and particle nucleation and growth process. The size and fluorescence switching properties of the NPs were systematically investigated in solution and in cel-lular uptake experiments. This work is anticipated to provide a simple and highly effective engineering strategy for the modulation of fluorescence switching nanoparticles and beneficial to its engineering application.(c) 2022 The Chemical Industry and Engineering Society of China, and Chemical Industry Press Co., Ltd. All rights reserved.