文献类型：期刊文献

英文题名：Orthogonal separation on one beta-cyclodextrin column by switching reversed-phase liquid chromatography and hydrophilic interaction chromatography

作者：Feng, Jia-tao[1];Guo, Zhi-mou[1];Shi, Hui[2];Gu, Jiang-ping[2];Jin, Yu[2];Liang, Xin-miao[1,2]

机构：[1]Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China;[2]E China Univ Sci & Technol, Engn Res Ctr Pharmaceut Proc Chem, Minist Educ, Sch Pharm, Shanghai 200237, Peoples R China

年份：2010

卷号：81

期号：4-5

起止页码：1870

外文期刊名：TALANTA

收录：;EI(收录号：20102012927315);WOS:【SCI-EXPANDED(收录号:WOS:000278737100113)】；

基金：This work was sponsored by National Natural Science Funds for Distinguished Young Scholars (20825518), Important National Science & Technology Specific Projects in the Eleventh Five-year Plan Period (2009ZX09313-003) and 111 Project (B07023).

语种：英文

外文关键词：Beta-cyclodextrin bonded stationary phase; Dual retention mechanism; Hydrophilic interaction chromatography; Reversed-phase liquid chromatography; Natural product

摘要：A dual retention combined with reversed-phase liquid chromatography (RP-LC) and hydrophilic interaction chromatography (HILIC) has been observed on beta-cyclodextrin (beta-CD) bonded stationary phase. A typical U-shaped retention curve was achieved owing to dual retention mechanism Based on this observation, a beta-CD column can be operated under reversed-phase liquid chromatography (RP-LC) and hydrophilic interaction chromatography (HILIC) modes. Two-dimensional liquid chromatography (2D-LC) analysis can be realized on Just a beta-CD column by switching these two different separation modes. In this study, off-line 2D-LC analysis for a natural product was carried out to prove the orthogonal separation between RP-LC and HILIC modes on a Click beta-CD column. Herba Hedyotis Diffusae, the whole grass of Hedyotis Diffusae wild was extracted with water, pretreated with macroporous resin and then first separated at RP-LC mode on the Click beta-CD column to obtain successive fractions, which were then reanalyzed at HILIC mode on the same Click beta-CD column The result proved that both separation modes on the Click beta-CD column have good retention and peak shape, and these two separation modes have good orthogonality 2D-LC analysis revealed abundant information in the natural product. Especially numerous minor components were enriched and separated. The mobile phase used in RP-LC and HILIC modes can be same and the switch between these two separation modes is easily realized by changing the ratio of the acetonitrile and water. Hence the mobile phase in this 2D-LC system is completely compatible. This advantage makes this combination is an appropriate 2D-LC method for the solutes having retention at both separation modes (C) 2010 Elsevier B V All rights reserved