文献类型：期刊文献

英文题名：A pre-column derivatization high-performance liquid chromatography method for simultaneous determination of short-chain and medium-chain fatty acids in a fecal sample

作者：Fu, Zhibo[1];Zhang, Hongyang[1];Zeng, Zhijun[2];Ning, Fanghong[3];Xu, Ziwei[4];Liu, Chenyu[1];Zhang, Min[5];Hu, Ping[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]Jiangxi Univ Chinese Med, Res Ctr Differentiat & Dev TCM Basic Theory, Jiangxi Prov Key Lab TCM Etiopathogenesis, Nanchang, Jiangxi, Peoples R China;[3]East China Univ Sci & Technol, Sch Biotechnol, Dept Biotechnol, Shanghai, Peoples R China;[4]Jiangxi Univ Chinese Med, Sch Pharm, Dept Pharmacol, Nanchang, Jiangxi, Peoples R China;[5]East China Univ Sci & Technol, Sch Pharm, China Shanghai Key Lab New Drug Design, Shanghai, Peoples R China

年份：2023

卷号：46

期号：1

外文期刊名：JOURNAL OF SEPARATION SCIENCE

收录：;EI(收录号：20224513090938);WOS:【SCI-EXPANDED(收录号:WOS:000878310200001)】；

基金：This research was funded by the National Natural Science Foundation of China (Grant number 81973285).

语种：英文

外文关键词：derivatization; feces sample; indole-3-acetic acid hydrazide; high-performance liquid chromatography; ultraviolet detector

摘要：Short-chain and medium-chain fatty acids have plentiful biological functions, which play a crucial role in the diagnosis and therapy of many diseases. Herein, a new method for simultaneous quantifying 17 short-chain and medium-chain fatty acids with high-performance liquid chromatography coupled with an ultraviolet detector was developed and the pre-column derivatization by indole-3-acetic acid hydrazide was performed to improve the separation and detection. The conditions of the derivatization reaction were systematically investigated. Subsequently, the method was validated and the results showed a satisfactory linearity (linear regression coefficients > 0.9969), the limit of detection (4.0x10(-3)-1.9x10(-2) mu mol/L), precision (0.9%-7.3% for intra-day and 2.0%-9.8% for inter-day), recovery (90.0%-109.1% with relative standard deviation <7.7%) and stability (0.1%-3.3% for standard solution and 0.2%-3.9% for fecal sample). Finally, the established method was successfully applied to quantify short-chain and medium-chain fatty acids in the feces of healthy control and diabetic rats. Eleven kinds of short-chain and medium-chain fatty acids were detected and six of them showed a significant difference between the control group and the model group.