文献类型：期刊文献

英文题名：Doped Graphene To Mimic the Bacterial NADH Oxidase for One- Step NAD plus Supplementation in Mammals

作者：Liu, Xi[1];Li, Jingkun[2];Zitolo, Andrea[3];Gao, Meng[1];Jiang, Jun[1];Geng, Xiangtian[4];Xie, Qianqian[1];Wu, Di[1];Zheng, Huizhen[1];Cai, Xiaoming[4];Lu, Jianmei[5];Jaouen, Frederic[6];Li, Ruibin[1]

机构：[1]Soochow Univ, Collaborat Innovat Ctr Radiol Med Jiangsu Higher E, Sch Radiol & Interdisciplinary Sci RAD X, Suzhou Med Coll,State Key Lab Radiat Med & Protect, Suzhou 215123, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[3]Synchrotron SOLEIL, Lorme Merisiers, F-91192 Gif Sur Yvette, France;[4]Soochow Univ, Suzhou Med Coll, Sch Publ Hlth, Suzhou 215123, Peoples R China;[5]Soochow Univ, Coll Chem Chem Engn & Mat Sci, Suzhou 215123, Peoples R China;[6]Univ Montpellier, ICGM, CNRS, ENSCM, F-34293 Montpellier, France

年份：2023

外文期刊名：JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000925748400001)】；

基金：This work was supported by grants from the National Key R&D Program of China, Ministry of Science and Technology of China (2020YFA0710700, 2022YFE0124000) , the National Natural Science Foundation of China (21976126) , the Natural Science Foundation of Jiangsu Province (BK20211545) , and the Project of National Center for International Research on Intelligent Nano-Materials and Detection Technology in Environmental Protection, Soochow University (No. SDGH2202) . We acknowledge Synchrotron SOLEIL (Gif-sur Yvette, France) for provision of synchrotron radiation facilities at beamline SAMBA (proposal number 20180635) .

语种：英文

摘要：Nicotinamide adenine dinucleotide (NAD) is a critical regulator of metabolic networks, and declining levels of its oxidized form, NAD+, are closely associated with numerous diseases. While supplementing cells with precursors needed for NAD+ synthesis has shown poor efficacy in combatting NAD+ decline, an alternative strategy is the development of synthetic materials that catalyze the oxidation of NADH into NAD+, thereby taking over the natural role of the NADH oxidase (NOX) present in bacteria. Herein, we discovered that metal-nitrogen-doped graphene (MNGR) materials can catalyze the oxidation of NADH into NAD+. Among MNGR materials with different transition metals, Fe-, Co-, and Cu-NGR displayed strong catalytic activity combined with >80% conversion of NADH into NAD+, similar specificity to NOX for abstracting hydrogen from the pyridine ring of nicotinamide, and higher selectivity than 51 other nanomaterials. The NOX-like activity of FeNGR functioned well in diverse cell lines. As a proof of concept of the in vivo application, we showed that FeNGR could specifically target the liver and remedy the metabolic flux anomaly in obesity mice with NAD+-deficient cells. Overall, our study provides a distinct insight for exploration of drug candidates by design of synthetic materials to mimic the functions of unique enzymes (e.g., NOX) in bacteria.