文献类型：期刊文献

英文题名：Microalbuminuria sensitive near-infrared AIE probe for point-of-care evaluating kidney diseases

作者：Zhu, Zhirong[1,2];Chen, Xiaoyan[3];Liao, Hongze[4];Li, Li[5];Yang, Haojian[1,2];Wang, Qi[1,2,6,7];Zhu, Wei-Hong[1,2,6,7]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Key Lab Adv Mat, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Inst Fine Chem, Sch Chem & Mol Engn, Joint Int Res Lab Precis Chem & Mol Engn,Feringa N, Shanghai, Peoples R China;[3]Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Dept Pathol, Shanghai, Peoples R China;[4]Shanghai Jiao Tong Univ, Ren Ji Hosp, Res Ctr Marine Drugs, Sch Med,Dept Pharm, Shanghai, Peoples R China;[5]Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Dept Lab Med, Shanghai, Peoples R China;[6]East China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[7]East China Univ Sci & Technol, Inst Fine Chem, Feringa Nobel Prize Scientist Joint Res Ctr, Joint Int Res Lab Precis Chem & Mol Engn,Frontiers, Shanghai 200237, Peoples R China

年份：2024

卷号：5

期号：3

外文期刊名：AGGREGATE

收录：EI(收录号：20241015699199);WOS:【ESCI(收录号:WOS:001175623000001)】；

基金：This work was supported by the National Key Research and Development Program of China (2021YFA0910000), NSFC (22222803, 91959202 and 21974047), China Postdoctoral Science Foundation (2022M72142), and Shanghai Municipal Science and Technology Major Project (2018SHZDZX03).

语种：英文

外文关键词：AIE-active probe; kidney disease; microalbuminuria; point-of-care testing

摘要：Urinary microalbumin (mALB) serves as an exceptionally sensitive indicator for the early detection of kidney damage, playing a pivotal role in identifying chronic renal failure and kidney lesions in individuals. Nevertheless, the current fluorescent methodologies for point-of-care (POC) diagnosis of mALB in real urine still exhibit suboptimal performance. Herein, the development and synthesis of QM-N2, an albumin-activated near-infrared (NIR) aggregation-induced emission (AIE) fluorescent probe, are presented. The strategic incorporation and positioning of quaternary ammonium salts within the quinoline-malononitrile (QM) scaffold significantly influence solubility and luminescence characteristics. Specifically, the quaternary ammonium salt-free variant, QM-OH, and the quaternary ammonium salt integrated at the donor function group (DFG) site, QM-N1, display limited solubility in aqueous solutions while demonstrating a distinct fluorescence signal. Conversely, the incorporation of quaternary ammonium salt at the conformational functional group (CFG) site in QM-N2 imparts superior dispersibility in water and reduces the initial fluorescence. Furthermore, the integration of a well-defined D-pi-A structure within QM-N2 enables itself with near-infrared emission, which is crucial for mitigating interference from autofluorescence present in urine samples. Upon interaction with albumin, QM-N2 forms a tight bond with the IIA site of the subdomain of human serum albumin (HSA), inducing alterations in protein configuration and constraining the intrinsic motion of fluorescent molecules. This interaction induces fluorescence, facilitating the sensitive detection of trace albumin. Ultimately, QM-N2 is applied for POC testing of mALB using portable equipment, particularly in the diagnosis of mALB-related diseases, notably chronic renal failure. This positioning underscores its potential as an ideal candidate for self-health measurement at home or in community hospitals. An albumin-activated near-infrared (NIR) aggregation-induced emission (AIE) fluorescent probe QM-N2 is designed, aiming to achieve rapid and accurate fluorescence detection of urinary microalbumin. Through a comprehensive analysis of numerous human urine samples, the fluorescence enhancement exhibited a robust correlation with the concentration of urinary microalbumin, positioning QM-N2 as a promising tool for the diagnosis of mALB-related kidney diseases. image