文献类型：期刊文献

中文题名：毕赤酵母碳源阻遏因子编码基因PpMIG1和PpMIG2的克隆与序列分析

英文题名：Cloning and Sequence Analysis of the Genes PpMIG1 and PpMIG2 Encoding Catabolite Repressors in Pichia pastoris

作者：张平[1];周祥山[1];张元兴[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2010

卷号：26

期号：11

起止页码：223

中文期刊名：生物技术通报

外文期刊名：Biotechnology Bulletin

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD_E2011_2012】；

基金：上海市重点学科建设项目(B505)

语种：中文

中文关键词：毕赤酵母;碳源阻遏因子;克隆

外文关键词：Pichia pastoris Catabolite repressor Cloning

摘要：通过MIG基因的同源性设计简并引物,采用PCR方法从毕赤酵母(Pichia pastoris)中成功克隆了两个MIG同源基因的核心片段,同时利用Genome Walking的方法获得了基因的全长及其侧翼序列,并分别命名为PpMIG1和PpMIG2。序列分析显示,PpMIG1基因全长1335bp,编码444个氨基酸;PpMIG2基因全长1365bp,编码454个氨基酸。这个两个基因所编码的蛋白质均与酿酒酵母碳源阻遏因子ScMig1同源,在氨基末端具有两个典型的C2H2锌指结构域,该结构域能够与受葡萄糖阻遏的许多基因的启动子相结合。PpMig阻遏因子的获得为毕赤酵母碳源阻遏机制的研究奠定了基础。
In this study,we have cloned two MIG1 homologous genes in Pichia patoris based on degenerate PCR and Genome Walking method.The two genes have been designated PpMIG1 and PpMIG2,respectively.Sequence analysis of the genes revealed a 1 335 bp open reading frame coding for 344 amino acids in the case of PpMIG1,and a 1 365 bp open reading frame for 354 amino acids in the case of PpMIG2.Both Pp MIG genes encode protein homologous to the DNA-binding repressor Mig1 from Saccharomyces cerevisiae（Sc Mig1）,which is a C2H2 zinc finger protein and could bind to the promoters of many genes repressed by glucose.The finding of these two catabolite repressors is the first step in elucidating the mechanisms of catabolite repression in P.pastoris.