文献类型：期刊文献

英文题名：Highly enantioselective oxidation of racemic phenyl-1,2-ethanediol to optically pure (R)-(-)-mandelic acid by a newly isolated Brevibacterium lutescens CCZU12-1

作者：He, Yu-Cai[1,2,3];Ma, Cui-Luan[1];Zhang, Xian[3];Li, Liang[1];Xu, Jian-He[2];Wu, Miao-Xin[1]

机构：[1]Changzhou Univ, Coll Pharmaceut & Life Sci, Biochem Engn Lab, Changzhou 213164, Peoples R China;[2]Jiangsu Key Lab Adv Catalyt Mat & Technol, Changzhou 213164, Peoples R China;[3]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2013

卷号：97

期号：16

起止页码：7185

外文期刊名：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000322408100011)】；

基金：This work was financially supported by the National Natural Science Foundation of China (No. 21102011), the Priority Academic Program Development of Jiangsu Higher Education Institutions, the Open Project Program of the State Key Laboratory of Bioreactor Engineering, Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology (No. BM2012110), and the Key Laboratory of Guangxi Biorefinery (No. GXBF11-03).

语种：英文

外文关键词：(R)-(-)-Mandelic acid; Phenyl-1,2-ethanediol; Brevibacterium lutescens CCZU12-1; Biotransformation; Enantioselective oxidation

摘要：Enantioselective oxidation of racemic phenyl-1,2-ethanediol into (R)-(-)-mandelic acid by a newly isolated Brevibacterium lutescens CCZU12-1 was demonstrated. It was found that optically active (R)-(-)-mandelic acid (e.e.p > 99.9 %) is produced leaving the other enantiomer (S)-(+)-phenyl-1,2-ethanediol intact. Using fed-batch method, a total of 172.9 mM (R)-(-)-mandelic acid accumulated in the reaction mixture after the seventh feed. Moreover, oxidation of phenyl-1,2-ethanediol using calcium alginate-entrapped resting cells was carried out in the aqueous system, and efficient biocatalyst recycling was achieved as a result of cell immobilization in calcium alginate, with a product-to-biocatalyst ratio of 27.94 g (R)-(-)-mandelic acid g(-1) dry cell weight cell after 16 cycles of repeated use.