文献类型：期刊文献

英文题名：Minor Phenolic Compounds in Detoxified Lignocellulosic Hydrolysates Are the Determinant Factor on Cell Growth and Metabolic Activity of Escherichia coli

作者：Wang, An[1];Han, Tao[2];Zhang, Bin[2];Bao, Jie[1,2]

机构：[1]Shanxi Univ, Sch Life Sci, Taiyuan, Shanxi, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China

年份：2025

卷号：20

期号：11

外文期刊名：BIOTECHNOLOGY JOURNAL

收录：;EI(收录号：20254619494071);WOS:【SCI-EXPANDED(收录号:WOS:001610406100001)】；

基金：This research was supported by the National Key R&D Program of China (2023YFA0914400), the National Natural Science Foundation of China (32301269), the Yangfan Project of Science and Technology Committee of Shanghai Municipality (23YF1409900), and the Key Technology R&D Program of the Science and Technology Commission of Shanghai Municipality (25HC2820200).

语种：英文

外文关键词：cadaverine synthesis; detoxification; Escherichia coli; ethanol fermentation; phenolic compounds; lignocellulose

摘要：Phenolic compounds from lignin degradation in harsh pretreatment of lignocellulose persistently exist at low concentrations in hydrolysates even after standard detoxification and enzymatic hydrolysis. This study found that minor phenolic compounds in dry acid-pretreated and biodetoxified wheat straw hydrolysates were the key inhibitors of Escherichia coli (E. coli). Multiple E. coli strains exhibited poor cell growth and metabolic activity in wheat straw hydrolysates with the presence of minor phenolics. However, the cell growth and metabolic activity of E. coli strains were significantly recovered with the declining phenolic content. Several recombinant E. coli strains successfully fermented 22.17 g/L of ethanol and synthesized 12.04 g/L of cadaverine using wheat straw hydrolysates after the removal of minor phenolics. These findings highlight the critical role of phenolic compounds in the inhibition of E. coli strains and provide a foundation for E. coli recombinants in biorefinery fermentations for biofuel and biochemical productions.