文献类型：期刊文献

中文题名：采用CFD技术优化倾斜式重力沉降细胞截留装置的结构

英文题名：Optimize Inclined Gravitational Settler with CFD for Animal Cells Perfusion Culture

作者：张璐[1];魏玮[1];张旭[1];周燕[1];谭文松[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2008

卷号：28

期号：S1

起止页码：178

中文期刊名：中国生物工程杂志

外文期刊名：China Biotechnology

收录：CSTPCD;;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：上海市自然科学基金资助项目(05ZR14037)

语种：中文

中文关键词：计算流体力学;灌注培养;重力沉降;选择截留

外文关键词：CFD;;Perfusion culture;;Gravitational settlement;;Selectively retention

摘要：倾斜式重力沉降细胞截留装置(简称沉降器)应用在大规模长期灌注培养过程中具有一定的优势,但是由于细胞容易在其内大量沉积,限制了其进一步的推广与应用。本文结合CFD技术和细胞沉积效果实验研究了沉降器内部的三维流场特性及其对细胞沉积量的影响。研究结果表明在细胞截留过程中,沉降器中细胞悬滞量远大于其壁面粘附量。以降低边界层厚度为目标进一步优化装置的结构参数,可以有效降低细胞的悬滞和粘附量,提高了倾斜式重力沉降截留装置的细胞截留效率。
The inclined gravitational settler showed some advantages in animal cells perfusion culture process.However,the further application of this setder had been limited because the cells were easy to deposit on its bottom.The properties of three-dimensional flow field inside the settler,which would influence the cells deposition,was investigated through the methods of combination of CFD technology and cold model experiments.The result showed that the amount of cells hung in the boundary layer were larger than that of adhered on the bottom.One of the alternative methods for this problem was to optimize setder structure by reducing the side-effect of boundary layer.With the result demonstrated that the amount of cells deposition could be reduced effectively in this optimized setder.