文献类型：期刊文献

英文题名：The QseE-QseF two-component system: A key mediator of epinephrine-regulated virulence in the marine pathogen Edwardsiella piscicida

作者：Ma, Jiabao[1];Ahmed, Moamer A. H.[1];Shao, Shuai[1,4];Zhang, Yuanxing[1,3,4];Wang, Qiyao[1,4,5];Yin, Kaiyu[2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Business Sch, Sch Hospitality Management, Shanghai 200235, Peoples R China;[3]Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai 519000, Peoples R China;[4]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai 200237, Peoples R China;[5]Shanghai Haosi Marine Biotechnol Co Ltd, Shanghai, Peoples R China

年份：2024

卷号：279

外文期刊名：MICROBIOLOGICAL RESEARCH

收录：;EI(收录号：20234915179529);WOS:【SCI-EXPANDED(收录号:WOS:001135121600001)】；

基金：This work was supported by grants from the National Natural Science Foundation of China (32102852) , the Natural Science Foundation of Shanghai (23ZR1445800) , and the Shanghai Municipal Science and Technology Commission Technical Leader Project (21XD1431900) .

语种：英文

外文关键词：Edwardsiella piscicida; EsrB; QseE-QseF; Epinephrine; Virulence

摘要：Edwardsiella piscicida is a widespread pathogen that infects various fish species and causes massive hemorrhagic septicemia, resulting in significant property damage to the global aquaculture industry. Type III and VI secretion systems (T3/T6SS), controlled by the master regulator EsrB, are important virulence factors of E. piscicida that enable bacterial colonization and evasion from host immune clearance. In this study, we demonstrate that the QseE-QseF two-component system negatively regulated esrB expression by reanalysis of Tn-seq data. Moreover, the response regulator QseF directly bound to esrB promoter and inhibited the expression of T3/T6SS genes, especially in the presence of epinephrine. Furthermore, in response to the prompt increasing of epinephrine level, the host immune genes were delayed repressed and QseE-QseF timely inhibited the expression of T3/T6SS genes to evade immune clearance. In summary, this study enhances our understanding and knowledge of the condi-tional pathogenesis mechanism and virulence regulation network of E. piscicida.