文献类型：期刊文献

英文题名：Purification of compounds from Lignum Dalbergia Odorifera using two-dimensional preparative chromatography with Click oligo (ethylene glycol) and C18 column

作者：Feng, Jiatao[1]; Xiao, Yuansheng[1]; Guo, Zhimou[1]; Yu, Danhua[2]; Jin, Yu[2]; Liang, Xinmiao[1,2]

机构：[1] Dalian Institute of Chemical Physics, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, Dalian 116023, China; [2] Engineering Research Centre of Pharmaceutical Process Chemistry, Ministry of Education, School of Pharmacy, East China University of Science and Technology, Shanghai, China

年份：2011

卷号：34

期号：3

起止页码：299

外文期刊名：Journal of Separation Science

收录：EI(收录号：20110513636620)

语种：英文

外文关键词：Column chromatography - Ethylene glycol - Separation - Ethylene - Medicine - Polyols

摘要：Purification of compounds from traditional Chinese medicines (TCMs) is an important task for understanding the chemical composition of TCMs. However, it is difficult to obtain compounds with high enough purity for identification by NMR due to the complexity of TCMs in chemical composition. In this study, a two-dimensional purification method based on a Click oligo (ethylene glycol) column and a C18 column was developed to realize an orthogonal separation in preparative level for purifying compounds efficiently. The first dimensional preparation was performed on a Click oligo (ethylene glycol) column to simplify the sample into the fractions with good separation repeatability. On the first dimension, 7.2g sample was separated into 11 fractions with a recovery of 86% within 6h. A C18 column was taken as the second dimension to realize the high-performance separation and rapid preparation from the fractions collected from the first dimension. Eight compounds in fraction 6 and 2 compounds in fraction 8 were isolated and identified after optimizing the separation and collection parameters. This method is a high-efficient and orthogonal preparation method to improve the separation of a complex sample and increase the purity of the compounds, which benefits from the application of novel materials in the preparation and purification. Copyright ? 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.