文献类型：期刊文献

英文题名：Mass Spectrometry Imaging-Based Single-Cell Lipidomics Profiles Metabolic Signatures of Heart Failure

作者：Ren, Jie[1];Li, Hao-Wen[2,3];Chen, Liang[1];Zhang, Min[3];Liu, Yan-Xiang[1];Zhang, Bo-Wen[1];Xu, Rui[2];Miao, Yan-Yan[2];Xu, Xue-Mei[2,3];Hua, Xin[4];Sun, Xiao-Gang[1];Yu, Ru-Jia[3,5];Long, Yi-Tao[3,5];Hu, Sheng-Shou[1]

机构：[1]Chinese Acad Med Sci CAMS & Perking Union Med Coll, Fuwai Hosp, Natl Ctr Cardiovasc Dis, State Key Lab Cardiovasc Dis, Beijing 100037, Peoples R China;[2]Shanghai Jiao Tong Univ, Renji Hosp, Inst Mol Med, Sch Med,Shanghai Key Lab Nucle Acid Chem & Nanomed, Shanghai 200127, Peoples R China;[3]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[4]Southeast Univ, Sch Chem & Chem Engn, Jiangsu Engn Lab Smart Carbon Rich Mat & Device, Nanjing 211189, Peoples R China;[5]Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Analyt Chem Life Sci, Nanjing 210023, Peoples R China

年份：2023

卷号：6

外文期刊名：RESEARCH

收录：;EI(收录号：20231814030780);WOS:【SCI-EXPANDED(收录号:WOS:000993946200003)】；

基金： This work was supported by the National Natural Science Foundation of China (21834001, 21906054, and 82100377), Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences (2021-1-I2M-006), Beijing Nova Program (Z211100002121046), ZhiShan Scholar Program of Southeast University, and programs for high-level entrepreneurial and innovative talents introduction of Jiangsu Province.

语种：英文

外文关键词：Cardiology - Mammals - Metabolism - Metabolites - Plants (botany) - Quality control - Secondary ion mass spectrometry

摘要：Heart failure (HF), leading as one of the main causes of mortality, has become a serious public health issue with high prevalence around the world. Single cardiomyocyte (CM) metabolomics promises to revolutionize the understanding of HF pathogenesis since the metabolic remodeling in the human hearts plays a vital role in the disease progression. Unfortunately, current metabolic analysis is often limited by the dynamic features of metabolites and the critical needs for high-quality isolated CMs. Here, high-quality CMs were directly isolated from transgenic HF mice biopsies and further employed in the cellular metabolic analysis. The lipids landscape in individual CMs was profiled with a delayed extraction mode in time-of -flight secondary ion mass spectrometry. Specific metabolic signatures were identified to distinguish HF CMs from the control subjects, presenting as possible single-cell biomarkers. The spatial distributions of these signatures were imaged in single cells, and those were further found to be strongly associated with lipoprotein metabolism, transmembrane transport, and signal transduction. Taken together, we systematically studied the lipid metabolism of single CMs with a mass spectrometry imaging method, which directly benefited the identification of HF-associated signatures and a deeper understanding of HF-related metabolic pathways.