文献类型：期刊文献

英文题名：Enzymatic synthesis of L-ascorbyl linoleate in organic media

作者：Song, QingXun[1]; Zhao, Ying[1]; Xu, WeiQin[1]; Zhou, WenYu[1]; Wei, DongZhi[1]

机构：[1]E China Univ Sci & Technol, New Word Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2006

卷号：28

期号：4

起止页码：211

外文期刊名：BIOPROCESS AND BIOSYSTEMS ENGINEERING

收录：;EI(收录号：2006109753416);WOS:【SCI-EXPANDED(收录号:WOS:000235753900001)】；

语种：英文

外文关键词：L-ascorbyl linoleate; immobilized lipase; organic solvents; enzymatic synthesis; transesterification

摘要：A novel L-ascorbyl fatty acid ester, L-ascorbyl linoleate was successfully prepared by enzymatic esterification and transesterification in a non-aqueous medium using immobilized lipase as biocatalyst. Changes in enzymatic activity and product yield were studied for the following variable: the nature of the fatty acid, the fatty acid concentration and water content. The yield of synthesis for the C18 unsaturated fatty acids were higher than for the C18 saturated fatty acid. Initial enzyme concentration does not affect the equilibrium of the reaction. And the product yield (33.5%) in the transesterification was higher than that of the esterification (21.8%) at a high-substrate concentration 0.3 M. The medium water content was found to have a distinct influence on the L-ascorbyl linoleate synthesis.