文献类型：期刊文献

英文题名：Feeding with poly(I:C) induced long-term immune responses against bacterial infection in turbot (Scophthalmus maximus)

作者：He, Jing[1];Wang, Zhuang[1];Zhao, Yanbo[1];Yang, Jin[1];Zhang, Yuanxing[2,3];Liu, Qin[1,3];Yang, Dahai[1,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai 519000, Peoples R China;[3]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai 200237, Peoples R China

年份：2021

卷号：2

外文期刊名：FISH AND SHELLFISH IMMUNOLOGY REPORTS

收录：WOS:【ESCI(收录号:WOS:001007678800033)】；

基金：This work was supported by the Natural Science Foundation of Shanghai (20ZR1415500), the Innovation Group Project of Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai) (311020005), and the Fundamental Research Funds for the Central Universities (222201211729).

语种：英文

外文关键词：Poly(I:C); Anti-oxidant stress; Intestinal immune responses; Bacterial-infection; turbot

摘要：Poly(I:C) is a kind of chemosynthetic double-stranded RNA (dsRNA) analogue which could act as TLR3 agonist and induce IFN production. It is widely applied in anti-virus treatment and immunoregulation, as well as vaccine adjuvant in farmed animals. However, whether poly(I:C) could activate innate immune response to defense against bacterial infection remains unclear. In this study, we established a feeding trial model with different dose of poly(I:C) in turbot larvae, then challenged with Edwardsiella piscicida after 3-7 weeks resting period. The results show that feeding turbot with poly(I:C) exhibited a stronger inflammatory response and antioxidant stress ability, and significantly elevated the survival rate within the decreased bacterial loads. Importantly, the bacterial infection-induced white feces in hindgut of turbot were significantly alleviated after poly(I:C) feeding, and this administration induced protection could last for about 7 weeks. Taken together, these findings indicate that feeding turbot with poly(I:C) could enhance a long-term intestinal mucosal immunity in response to bacterial infection, suggesting that poly(I:C) might be a promising immunostimulant in aquaculture.