文献类型：期刊文献

中文题名：Mass Spectrometry Imaging-Based Single-Cell Lipidomics Profiles Metabolic Signatures of Heart Failure

作者：Jie Ren[1];Hao-Wen Li[2,3];Liang Chen[1];Min Zhang[3];Yan-Xiang Liu[1];Bo-Wen Zhang[1];Rui Xu[2];Yan-Yan Miao[2];Xue-Mei Xu[2];Xin Hua[3,4];Xiao-Gang Sun[1];Ru-Jia Yu[3,5];Yi-Tao Long[3,5];Sheng-Shou Hu[1]

机构：[1]State Key Laboratory of Cardiovascular Disease,Fuwai Hospital,National Center for Cardiovascular Disease,Chinese Academy of Medicine Science(CAMS)and Perking Union Medical College(PUMC),Beijing,100037,P.R.China;[2]Institute of Molecular Medicine,Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine,State Key Laboratory of Oncogenes and Related Genes,Renji Hospital,School of Medicine,Shanghai Jiao Tong University,Shanghai,200127,P.R.China;[3]School of Chemistry and Molecular Engineering,East China University of Science and Technology,Shanghai,200237,P.R.China;[4]Jiangsu Engineering Laboratory of Smart Carbon-Rich Materials and Device,School of Chemistry and Chemical Engineering,Southeast University,Nanjing,211189,P.R.China;[5]State Key Laboratory of Analytical Chemistry for Life Science,School of Chemistry and Chemical Engineering,Nanjing University,Nanjing,210023,P.R.China

年份：2023

期号：3

起止页码：309

中文期刊名：Research

外文期刊名：研究（英文）

收录：CSTPCD;;Scopus;CSCD:【CSCD2023_2024】；

基金：the National Natural Science Foundation of China(21834001,21906054,and 82100377);Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences(2021-1-12M-006);Beijing Nova Program(Z211100002121046),ZhiShan Scholar Program of Southeast University,and programs for high-level entrepreneurial and innovative talents introduction of Jiangsu Province.

语种：英文

中文关键词：signature;metabolism;Profiles;

摘要：Heart failure(HF),leading as one of the main causes of mortality,has become a serious public health issue with high prevalence around the world.Single cardiomyocyte(CM)metabolomics promises to revolutionize the understanding of HF pathogenesis since the metabolic remodeling in the human hearts plays a vital role in the disease progression.Unfortunately,current metabolic analysis is often limited by the dynamic features of metabolites and the critical needs for high-quality isolated CMs.Here,high-quality CMs were directly isolated from transgenic HF mice biopsies and further employed in the cellular metabolic analysis.The lipids landscape in individual CMs was profiled with a delayed extraction mode in time-of-flight secondary ion mass spectrometry.Specific metabolic signatures were identified to distinguish HF CMs from the control subjects,presenting as possible single-cell biomarkers.The spatial distributions of these signatures were imaged in single cells,and those were further found to be strongly associated with lipoprotein metabolism,transmembrane transport,and signal transduction.Taken together,we systematically studied the lipid metabolism of single CMs with a mass spectrometry imaging method,which directly benefited the identification of HF-associated signatures and a deeper understanding of HF-related metabolic pathways.