文献类型：期刊文献

中文题名：高速逆流色谱法分离灵芝中的灵芝烯酸B

英文题名：Separation of ganoderenic acid B from Ganoderma lingzhi by high-speed countercurrent chromatography

作者：岳亚文[1,2];钱丁寅[2];韩伟[2];王晨光[3];冯杰[1];唐庆九[1];张忠[1];冯娜[1];张劲松[1]

机构：[1]上海市农业科学院食用菌研究所国家食用菌工程技术研究中心农业部南方食用菌资源利用重点实验室上海市农业遗传育种重点开放实验室,上海201403;[2]华东理工大学药学院制药工程与过程化学教育部工程研究中心上海市新药设计重点实验室,上海200237;[3]上海百信生物科技有限公司,上海201403

年份：2020

卷号：39

期号：1

起止页码：193

中文期刊名：菌物学报

外文期刊名：Mycosystema

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：上海市科学技术委员会科研计划项目(17391903300)~~

语种：中文

中文关键词：药用真菌;三萜酸;工艺优化

外文关键词：medicinal fungi;triterpenoid acid;process optimization

摘要：本实验建立一种快速从灵芝子实体中制备灵芝烯酸B的方法。以沪农灵芝1号子实体乙醇提取物为原料,经过D101大孔树脂粗分,用60%乙醇洗脱后,采用高速逆流色谱对获得的灵芝酸性三萜进行分离制备。确定最佳溶剂体系为正己烷-乙酸乙酯-甲醇-水(V/V/V/V,5:5:2:9),上相作为固定相,下相作为流动相,单因素法和正交实验设计确定高速逆流色谱法分离灵芝烯酸B的最佳工艺为:流速2.0mL/min,转速900r/min,一次上样量为500mg时,制备得到灵芝烯酸B化合物得率为(9.07±0.16)%,纯度为(85.04±3.45)%。用质谱、核磁对得到的灵芝烯酸B进行了结构鉴定。此法操作简单高效,为大量制备灵芝烯酸B提供了参考。
A rapid method for isolation of ganoderenic acid B from fruiting bodies of Ganoderma lingzhi is described.The extract of ganoderma triterpenoid acids(GTA)was obtained from G.lingzhi Hunong No.1 by using D101 macroporous resin as filler and the extract was eluted by 60%ethanol.The separation of obtained GTA sample was performed by high-speed countercurrent chromatography(HSCCC).The optimal solvent system was determined to be hexane-ethyl acetate-methanol-water(V/V/V/V,5:5:2:9)with the upper-layer as stationary phase and the sub-layer as mobile phase.The single-factor experiments and orthogonal experiments were carried out to determine the optimal processing for the preparation of ganoderenic acid B.Flow rate of 2.0 mL/min,rotational speed of 900 r/min,and loading quantity of 500 mg were proved to be optimal for the preparation process,and the yield of ganoderenic acid B reached(9.07±0.16)%and the purity reached(85.04±3.45)%.The structure of ganoderenic acid B was identified by nuclear magnetic resonance(NMR)and mass spectroscopy.This method is simple and efficient,and suitable to large scale preparation of ganoderenic acid B.