文献类型：期刊文献

英文题名：A Bacteria-infected-microenvironment-triggered self-adaptive protein-binding nano-inhibitor for photodynamic elimination of drug-resistant biofilms

作者：Wang, Wenchen[1];Wang, Anan[1];Huang, Wenlong[1];Zhang, Zhiqiang[1];Li, Weiheng[1];Zhang, Mingming[2];Zhang, Weian[1];Tian, Jia[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]Chinese Acad Med Sci & Peking Union Med Coll, Tianjin Key Lab Biomed Mat, Inst Biomed Engn, Tianjin 300192, Peoples R China

年份：2026

卷号：705

外文期刊名：JOURNAL OF COLLOID AND INTERFACE SCIENCE

收录：;EI(收录号：20254819595106);WOS:【SCI-EXPANDED(收录号:WOS:001631108500001)】；

基金：This work was financially supported by the National Natural Science Foundation of China (Nos. 22575086, 22375218, and 52333014) and the Science and Technology Commission of Shanghai Municipality (No. 24520713200) .

语种：英文

外文关键词：Photodynamic therapy; Protein-binding; RAFT polymerization; Porphyrin

摘要：Photodynamic therapy (PDT) is a promising antibacterial and biofilm disrupting strategy to overcome the global escalation of antimicrobial resistance. However, the short lifespan, narrow diffusion ranges, and no target specificity of the reactive oxygen species (ROS) generated in PDT significantly limit the antibacterial and biofilmeliminated efficiency. Herein, we present a multifunctional nano-inhibitor with protein-binding and bacteria-infected microenvironment (BME) activated ROS generation for precise and efficient photodynamic elimination of drug-resistant biofilms. Under the acidic BME, the protonation of nano-inhibitors leads to self-expansion and exposure of targeting ligands, contributing to subsequent bacteria-and protein-binding via electrostatic interaction and disulfide covalent linkages. The shortened distance between proteins and nano-inhibitors thereby significantly enhanced oxidative damage, protein denaturation, and bactericidal performance. Both in vitro and in vivo studies demonstrated that the nano-inhibitors exhibited BME-triggered efficient antibacterial activity, biofilm elimination, and outstanding biocompatibility. The protein-binding PDT strategy provides a promising pathway for combating drug-resistant bacteria and biofilms.