文献类型：期刊文献

英文题名：Preliminary application of light-pH sensitive recycling aqueous two-phase systems to purification of lipase

作者：Li, Xian[1];Wan, Junfen[1];Cao, Xuejun[1]

机构：[1]E China Univ Sci & Technol, Dept Biochem Engn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2010

卷号：45

期号：4

起止页码：598

外文期刊名：PROCESS BIOCHEMISTRY

收录：;EI(收录号：20101012749397);WOS:【SCI-EXPANDED(收录号:WOS:000276376900025)】；

基金：This project was supported by Natural Scientific Foundation of China (20474016), and the National Special Fund for State Key Laboratory of Bioreactor Engineering (2060204). Authors very appreciate for above National funds.

语种：英文

外文关键词：pH-sensitive polymer; Light-sensitive polymer; Aqueous two-phase systems; Purification; Recovery; Lipase

摘要：One key problem of aqueous two-phase systems (ATPS) is that phase-forming polymers could not be recycled efficiently. This results in high cost and environmental pollution. In this study, we introduced novel aqueous two-phase systems which are composed by pH-sensitive polymer P-ADB and light-sensitive polymer P-NNC. P-NNC is enriched in the top phase while P-ADB is found in the bottom phase. And recoveries of two-phase-forming polymers can both reach over 96%. This aqueous two-phase system was used for purification of lipase from its crude material. The influences of various process parameters such as concentration of the phase-forming polymer, system pH. different types and concentrations of neutral salts on partitioning of lipase are evaluated. It has been found that partition coefficient of pure lipase could reach 0.061 under optimized conditions. Lipase from crude material was purified with 83.7% recovery and a purification factor of approximately 18 folds. (C) 2009 Elsevier Ltd. All rights reserved.