文献类型：期刊文献

英文题名：Mussel-inspired injectable hydrogel and its counterpart for actuating proliferation and neuronal differentiation of retinal progenitor cells

作者：Tang, Zhimin[1,2];Jiang, Fang[3];Zhang, Yuanhao[3];Zhang, Yi[1,2];Yang, Yuan[1,2];Huang, Xiaolin[1,2];Wang, Yuyao[1,2];Zhang, Dandan[1,2];Ni, Ni[1,2];Liu, Feng[3];Luo, Min[1,2];Fan, Xianqun[1,2];Zhang, Weian[3];Gu, Ping[1,2]

机构：[1]Shanghai Jiao Tong Univ, Sch Med, Peoples Hosp 9, Dept Ophthalmol, Shanghai 200011, Peoples R China;[2]Shanghai Key Lab Orbital Dis & Ocular Oncol, Shanghai 200011, Peoples R China;[3]East China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China

年份：2019

卷号：194

起止页码：57

外文期刊名：BIOMATERIALS

收录：;EI(收录号：20190206361021);WOS:【SCI-EXPANDED(收录号:WOS:000457504900006)】；

基金：The research was supported by the National Natural Science Foundations of China (81570883, 81870687 and 21574039), the National Key R&D program of China (2018YFC1106101), Shanghai Municipal Education Commission-Gaofeng Clinical Medicine Grant Support (20161316) and the Science and Technology Commission of Shanghai (17DZ2260100).

语种：英文

外文关键词：Hydrogel; Retinal progenitor cells; Polydopamine; Proliferation; Differentiation

摘要：Biomaterials-mediated retinal progenitor cell (RPC)-based transplantation therapy has shown substantial potential for retinal degeneration (RD), but it is limited by the poor RPC survival, proliferation and differentiation. Herein, the gelatin-hyaluronic acid (Gel-HA)-based hydrogels formed via moderate Michael-type addition reaction with or without the introduction of mussel-inspired polydopamine (PDA), i.e. Gel-HA-PDA and its counterpart Gel-HA hydrogels are developed, and their effects on the biological behaviour of RPCs, including adhesion, survival, proliferation, differentiation, delivery and migration are investigated. The hybrid hydrogels can adopt the intricate structure of the retina with suitable mechanical strength, degradation rate and biological activity to support cellular adhesion, survival and delivery. Meanwhile, Gel-HA hydrogel can remarkably promote RPC proliferation with much larger cell clusters, while Gel-HA-PDA hydrogel significantly enhances RPC adhesion and migration, and directs RPCs to preferentially differentiate toward retinal neurons such as photoreceptors (the most crucial cell-type for RD treatment), which is mainly induced by the activation of integrin alpha 51 beta-phosphatidylinositol-3-kinase (PI3K) pathway. This study demonstrates that Gel-HA hydrogel possesses great potential for RPC proliferation, while mussel-inspired PDA-modified Gel-HA hydrogel with superior bio-compatibility can significantly promote RPC neuronal differentiation, providing new insights for developing biomedical materials applied for RPC-based transplantation therapy.