文献类型：期刊文献

英文题名：Nutrition and bioprocess development for efficient biosynthesis of an antitumor compound from marine-derived fungus

作者：Zhou, Weiqiang[1];Cai, Menghao[1];Zhou, Jiushun[1];Jiang, Tao[1];Zhou, Jiao[1];Wang, Meixia[1];Zhou, Xiangshan[1];Zhang, Yuanxing[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2013

卷号：40

期号：10

起止页码：1131

外文期刊名：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

收录：;EI(收录号：20242616467476);WOS:【SCI-EXPANDED(收录号:WOS:000324234100005)】；

基金：This work was financially supported by the Chinese National High Technology Research and Development Program (2011AA090702, 2012AA092103, 2012AA092105) and National Natural Science Funds of China (31270141). We thank Prof. Zhigang She, Sun Yat-Sen University, for supplying the strain and Dr. Ke Na, Shanghai Institute of the Pharmaceutical Industry, for supplying the 1403C standard.

语种：英文

外文关键词：Halorosellinia sp (no. 1403); Medium optimization; 1403C; Fermentation process; Antitumor

摘要：An integrated nutrition and bioprocess strategy was developed for improving the biosynthesis of an antitumor compound, 1403C, by a marine-derived fungus, Halorosellinia sp. (no. 1403). First, statistical design strategies were synthetically applied to optimize the nutritional composition. The resulting 1403C production reached 2.07 g/l, which was 143.5 % higher than the original production. However, it only produced 0.44 g/l of 1403C in 5-l bioreactor fermentation. Thus, the operating parameters including culture pH, dissolved oxygen, agitation speed, impeller type and inoculum level were considered to improve the fermentation process, and an effective control strategy for 1403C production by Halorosellinia sp. submerged in a 5-l bioreactor was established. When inoculating 0.22 g/l dry biomass, controlling dissolved oxygen not lower than 30 % during the growth phase but ranging between 30 and 40 % during the stationary phase, using a double-layer six-flat-blade Rushton disc turbine agitated at 400 rpm, keeping short-term low pH and rapid-rising pH with glucose starvation, the highest 1403C production was finally obtained at 1.32 g/l, which was promoted by 200 % compared to before optimization. Fermentation scale-up was finally performed in a 500-l bioreactor, and 1403C production of 1.09 g/l was obtained.