文献类型：期刊文献

英文题名：Expression and purification of exendin-4, a GLP-1 receptor agonist, in Escherichia coli

作者：Yin, XP; Wei, DZ; Yi, LN; Tao, XY; Ma, YS

机构：[1]E China Univ Sci & Technol, Inst Biochem, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2005

卷号：41

期号：2

起止页码：259

外文期刊名：PROTEIN EXPRESSION AND PURIFICATION

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000229192800004)】；

语种：英文

外文关键词：exendin-4; expression; glucose-lowering action

摘要：Exendin-4 is a 39 amino acid peptide isolated from salivary secretions of Gila monster (Heloderma suspectrum). It shows 53% sequence similarity to glucagon-like peptide-1 (GLP-1), which is evaluated for the regulation of plasma glucose in type 2 diabetes. Exendin-4 is a potent and long-acting agonist of GLP-1 receptor. In the present Study, the exendin-4 gene obtained by PCR with an enterokinase site at N-terminus and a termination codon at C-terminus was expressed in Escherichia coli strain BL21 (DE3) harboring pET32a(+). The fusion protein was purified by chromatography on Ni-NTA-agarose column. Recombinant exendin-4 was obtained by enterokinase cleavage of the fusion protein and subsequent purification. The yield of recombinant exendin-4 was 3.15 mg/10g bacteria. The obtained recombinant exendin-4 shows glucose-lowering action in vivo. (c) 2004 Elsevier Inc. All rights reserved.