文献类型：期刊文献

英文题名：Characterization of Enzymes in the Oxidation of 1,2-Propanediol to d-(-)-Lactic Acid by Gluconobacter oxydans DSM 2003

作者：Wei, Liujing[1];Yang, Xuepeng[2];Gao, Keliang[1];Lin, Jinping[1];Yang, Shengli;Hua, Qiang;Wei, Dongzhi[1]

机构：[1]E China Univ Sci & Technol, Newworld Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Zhengzhou Univ Light Ind, Coll Food & Biol Engn, Zhengzhou 450002, Peoples R China

年份：2010

卷号：46

期号：1

起止页码：26

外文期刊名：MOLECULAR BIOTECHNOLOGY

收录：;EI(收录号：20103213143213);WOS:【SCI-EXPANDED(收录号:WOS:000280641300003)】；

基金：This study was financially supported by The National Key Basic Research Development Program of China (973 Program NO. 2009CB724703), and partly supported by National Natural Science Foundation of China (No. 20976053/B060804), Shanghai Pujiang Program (No. 08PJ14038), and National Special Fund for State Key Laboratory of Bioreactor Engineering (No. 2060204).

语种：英文

外文关键词：Gluconobacter oxydans; Alcohol dehydrogenase; Aldehyde dehydrogenase; 1,2-Propanediol; D-(-)-lactic acid

摘要：Although Gluconobacter oxydans can convert 1,2-propanediol to d-(-)-lactic acid, the enzyme(s) responsible for the conversion has remain unknown. In this study, the membrane-bound alcohol dehydrogenase (ADH) of Gluconobacter oxydans DSM 2003 was purified and confirmed to be essential for the process of d-(-)-lactic acid production by gene knockout and complementation studies. A 25 percent decrease in d-(-)-lactic acid production was found for the aldehyde dehydrogenase (ALDH) deficient strain of G. oxydans DSM 2003, indicating that this enzyme is involved in the reaction but not necessary. It is the first report that reveals the function of ADH and ALDH in the biooxidation of 1,2-propanediol to d-(-)-lactic acid by G. oxydans DSM 2003.