文献类型：期刊文献

英文题名：Dependence of fungal characteristics on seed morphology and shear stress in bioreactors

作者：Lu, Hongzhong[1];Li, Chao[1];Tang, Wenjun[1];Wang, Zejian[1];Xia, Jianye[1];Zhang, Siliang[1];Zhuang, Yingping[1];Chu, Ju[1];Noorman, Henk[2]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]DSM Biotechnol Ctr, NL-2600 MA Delft, Netherlands

年份：2015

卷号：38

期号：5

起止页码：917

外文期刊名：BIOPROCESS AND BIOSYSTEMS ENGINEERING

收录：;EI(收录号：20153101100209);WOS:【SCI-EXPANDED(收录号:WOS:000351512700013)】；

基金：This work was financially supported by Royal DSM (Delft, the Netherlands) and partially supported by NWO-MoST Joint Program (2013DFG32630), National Basic Research Program (973 Program 2013CB733600), National High Technology Research and Development Program of China (863 Program 2012AA021201) and National Key Technology R&D Program (2012BAI44G01). We would like to thank Sybe Hartmans, Qing Yuan Yin and Jie Zhao for their kind concern on this project.

语种：英文

外文关键词：Aspergillus niger; Morphological control; Stirred tank; Mass transfer; Rheology; Hydrodynamics

摘要：The fungal morphology during submerged cultivations has a profound influence on the overall performance of bioreactors. In this research, glucoamylase production by Aspergillus niger has been taken as a model to improve more insights. The morphology engineering could be conducted effectively by changing the seed morphology, as well as specific power input. During the fed-batch cultivations, pellet formation under milder shear stress field helped to reduce the broth viscosity, thus relieving oxygen limitation and promoting the enzyme production. Furthermore, we found that the relation between the shear stress field, which was characterized by energy dissipation rate/circulation function (EDCF), and enzyme activity was consistent with quadratic parabola, which threw light on the process optimization and scale-up for industrial enzyme production.