文献类型：期刊文献

中文题名：纳米孔道单分子电化学信号在线识别与分析研究

英文题名：Real-time Event Recognition and Analysis System for Nanopore Study

作者：王慧锋[1];黄飞[1];顾震[2];胡正利[2];应佚伦[2];颜秉勇[1];龙亿涛[2]

机构：[1]华东理工大学信息科学与工程学院,上海200237;[2]华东理工大学化学与分子工程学院,结构可控先进功能材料及其制备重点实验室,上海200237

年份：2018

卷号：46

期号：6

起止页码：843

中文期刊名：分析化学

外文期刊名：Chinese Journal of Analytical Chemistry

收录：CSTPCD;;EI(收录号：20182405301449);Scopus;WOS:【SCI-EXPANDED(收录号:WOS:000438469800004)】；北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；

基金：This work was supported by the National Natural Science Foundation of China (Nos. 21327807, 21421004), the Program of Shanghai Subject Chief Scientist of China (No. 15XD1501200), and the Fundamental Research Funds for the Central Universities of China (Nos. 222201718001, 222201717003).

语种：中文

中文关键词：纳米孔道;实时采集存储;在线分析处理;双缓冲区;自适应阈值法

外文关键词：Nanopore;Data acquisition and storage;Real-time data analysis and processing;Data bull）ring;Adaptive threshold algorithm

摘要：为实现纳米孔道单分子检测中对微弱电流信号的快速精准处理,考察了纳米孔道实验数据的信号特征,提出了基于双缓冲数据结构和有限冲击响应滤波的实时自适应阈值法,并基于这一算法设计了纳米孔道信号在线识别与分析系统,实现了实验数据实时采集存储和信号在线分析处理的同步进行。为验证所建立的纳米孔道信号在线识别和分析系统性能,采用噪音为20~100 p A和带宽区间为3~100 k Hz的仿真信号进行信号识别分析。结果表明,本系统能够满足强噪声、低带宽、高采样率(250 k Hz)环境下对实验数据处理的要求。将此系统应用于单个poly(dA)_4分子的Aerolysin纳米孔道分析实验中,实验结果表明,本系统能够对大数据量的纳米孔道实验数据进行实时、快速、精准的分析处理。
To achieve fast and accurate analysis of weak current signal of nanopore-based single molecule detection,we designed a real-time adaptive threshold data processing algorithm with data buffering technique and finite impulse response filtering. The system,which is designed based on the data processing algorithm,could realize real-time recognition and analysis of nanopore events during the data recording process. In order to verify the performance of the system,the ideal signals with different noise level（ 20-100 p A） and recording bandwidth（ 3-100 k Hz） was generated. The results showed that the system was stable to analyze the generated signals even at high noise. In addition,the system was also suitable for the data recording conditions of low bandwidth and high sampling rate（ 250 k Hz）. The proposed nanopore data processing system was further applied in the Aerolysin nanopore experiment for the detection of poly（dA）_4 molecules. The results showed that the data processing system could be applied in real nanopore recording system with high accuracy and speed.