文献类型：期刊文献

英文题名：Design and evaluation of capillary electrophoresis in dynamically coated capillaries coupled with chemiluminescence detection

作者：Liu, Haiyan[1];Han, Ning[1];Zhang, Lingyi[1];Du, Yiping[1];Zhang, Weibing[1]

机构：[1]E China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China

年份：2010

卷号：680

期号：1-2

起止页码：48

外文期刊名：ANALYTICA CHIMICA ACTA

收录：;EI(收录号：20104313324398);WOS:【SCI-EXPANDED(收录号:WOS:000284385800007)】；

基金：The authors are grateful for financial support from the Excellent Youth Founding of East China University of Science and Technology (YJ0157104), National Natural Sciences Foundation of China (No. 20675083) and "973" program (2007CB914102). The authors also hope to thank Dr. Intisar for his helpful discussion in grammar and sentence construction in our manuscript.

语种：英文

外文关键词：Dynamic coating; Capillary electrophoresis; Interface; Chemiluminescence; Hemoglobin; Haemolysis

摘要：A dynamic coating capillary electrophoresis coupled with a simplified on-line chemiluminescence detection system was designed and evaluated. In the proposed system, poly-vinylpyrrolidone was used as dynamic coating substance in the separation buffer to reduce the unwanted protein non-specific adsorption, which was first applied in capillary electrophoresis coupling with on-line chemiluminescence detection. In order to avoid complex processing, an ordinary plastic cuvette was modified as a three-way joint. The chemiluminescence reaction conditions and capillary electrophoresis separation conditions were investigated in detail. The results showed that the coated capillary can be injected protein samples at least 30 times continuously with good repeatability. Under optimal conditions, the chemiluminescence relative intensity was linear with the concentration of hemoglobin in the range of 4-1850 mu g mL(-1) and the detection limit was 2.014 mu g mL(-1) (S/N = 3). The relative standard deviation of migration times and peak heights for 40 mu g mL(-1) hemoglobin were 2.5% and 4.1% (n = 11) respectively. Interference of matrix effects was overcome by the calibration according to standard addition methods. Afterwards, the method was validated successfully and was applied to detect the concentration of hemoglobin in the serum of haemolytic patients. (C) 2010 Elsevier B.V. All rights reserved.