文献类型：期刊文献

英文题名：In vitro macrophage uptake and in vivo biodistribution of long-circulation nanoparticles with poly(ethylene-glycol)-modified PLA (BAB type) triblock copolymer

作者：Shan, Xiaoqian[1];Liu, Changsheng[1,2];Yuan, Yuan[3];Xu, Feng[1];Tao, Xinyi[1];Sheng, Yan[3];Zhou, Huanjun[3]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Minist Educ, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, Minist Educ, Engn Res Ctr Biomed Mat, Shanghai 200237, Peoples R China

年份：2009

卷号：72

期号：2

起止页码：303

外文期刊名：COLLOIDS AND SURFACES B-BIOINTERFACES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000267729300020)】；

基金：The authors acknowledge the financial support from the National High Technology Research and Development Program of China (863 program) (No. 2004AA-302050) and from Shanghai Nanotechnology Special Foundation (No. 0452nm022).

语种：英文

外文关键词：Nanoparticles; BAB type copolymers; PEGylation degree; Uptake by macrophages; Plasma clearance; Biodistribution

摘要：The effect of the PEG-grafted degree in the range of 0-30% on the in vitro macrophage uptake and in vivo biodistribution of poly(ethylene glycol)-poly(lactic acid)-poly(ethylene glycol) (PELE) nanoparticles (NPs) were investigated in this paper. The prepared NPs were characterized in terms of size, zeta potential, hydrophilicity, poly(vinyl alcohol) (PVA) residual on nanoparticles surfaces as well as drug loading. The macrophage uptake and biodistribution including plasma clearance kinetics following intravenous administration in mice of the NPs labeled by 6-coumarin were evaluated. The results showed that, except for the particles size, the hydrophilicity, superficial charges and in vitro phagocytosis amount of NPs are dependent on the PEG content in the copolymers greatly. The higher of the PEG content, the more hydrophilicity and the nearer to neutral surface charge was observed. And the prolonged circulation half-life (t(1/2)) of the PELE NPs in plasma was also strongly depended on the PEG content with the similar trend. In particular for PELE30 (containing 30% of PEG content) NPs. with the lowest phagocytosis uptake accompanied the highest hydrophilicity and approximately neutral charge, it had the longest half-life in vivo with almost 12-fold longer and accumulation in the reticuloendothelial system organs close to 1/2-fold lower than those of reference PLA. These results demonstrated that the PELE30 NPs with neutral charge and suitable size has a promising potential as a long-circulating oxygen carrier system with desirable biocompatibility and biofunctionality. (C) 2009 Elsevier B.V. All rights reserved.