文献类型：期刊文献

英文题名：Dictated cell adhesion and migration using microfluidic-controlled synthetic hydrogels exhibiting programmable viscoelasticities

作者：Yang, Haochen[1];Li, Ziyuan[1];Davidson-Rozenfeld, Gilad[3];Li, Meng[1];Shang, Yuxing[2];Chen, Linjie[1];Ma, Yingchao[1];Ge, Yifan[2];Willner, Itamar[3];Zhang, Junji[1]

机构：[1]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Sch Chem & Mol Engn,Inst Fine Chem, Frontiers Sci Ctr Materiobiol & Dynam Chem,Key Lab, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Interdisciplinary Res Ctr Biol & Chem, Shanghai Inst Organ Chem, Shanghai 201210, Peoples R China;[3]Hebrew Univ Jerusalem, Inst Chem, Ctr Nanosci & Nanotechnol, IL-91904 Jerusalem, Israel

年份：2026

外文期刊名：JOURNAL OF MATERIALS CHEMISTRY B

收录：;EI(收录号：20261920661185);WOS:【SCI-EXPANDED(收录号:WOS:001756365300001)】；

基金：This work is supported by the National Key R&D Program of China (2023YFF0722600), NSFC (22122803, 22378121, 22105070, 32350018), Science and Technology Commission of Shanghai Municipality (24DX1400200), and the Fundamental Research Funds for the Central Universities (222201717003). JZ acknowledges Shanghai Natural Science Foundation Project (23ZR1479500, 23JC1401700). ZL acknowledges Shanghai Sailing Program (20YF1410300).

语种：英文

外文关键词：Binary mixtures - Cell adhesion - Cell engineering - Cell signaling - Cells - Crosslinking - Elastic moduli - Hyaluronic acid - Hydrogels - Matrix algebra - Microfluidics - Tissue

摘要：Mechanosensing interactions between the extracellular matrix (ECM) and the intracellular cytoskeleton are fundamental to cellular functions such as motility, proliferation, and adhesion, driven by the dynamic, bidirectional, tension-regulated maturation of focal adhesion (FA) sites. We demonstrate that native mechanosensing interactions and their downstream functions are precisely controlled using synthetic hydrogels. We introduce a microfluidic-assisted synthesis of imine-crosslinked hyaluronic acid-gelatin copolymer hydrogels (HAG), enabling controlled, predefined gradient viscoelasticity. Specifically, three native tissues (muscle, epidermis, and cartilage)-mimicking HAG hydrogels were prepared, matching their effective Young's modulus (Ymod) and stress relaxation time (tau 1/2). Enhanced cell spreading and directional cell migration are observed, with a preference for substrates with tissue-matching viscoelasticity. These mechanosensing reactions are confirmed by traction force microscopy, revealing a tight correlation between native tissue mechano-properties and the hydrogel viscoelastic parameters. We demonstrate that the signaling efficacies of the FAK and associated YAP/TAZ pathways, central regulators of FA formation and cell migration, are tuned by substrate tissue-matching viscoelasticity. We implement these preprogrammed viscoelastic gradient hydrogels as spatiotemporal cell-separation matrices, enabling viscoelasticity-driven migration of binary cell mixtures. This work provides a potent platform for studying cell-material interactions, offering significant potential applications in tissue engineering, immunotherapy, and regenerative medicine.