文献类型：期刊文献

英文题名：Synthesis of rosmarinic acid analogues in Escherichia coli

作者：Zhuang, Yibin[1,3];Jiang, Jingjie[1,2];Bi, Huiping[1,3];Yin, Hua[1,3];Liu, Shaowei[2];Liu, Tao[1,3]

机构：[1]Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China;[2]E China Univ Sci & Technol, Dept Food Sci & Technol, Sch Biol Engn, Shanghai 200237, Peoples R China;[3]Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China

年份：2016

卷号：38

期号：4

起止页码：619

外文期刊名：BIOTECHNOLOGY LETTERS

收录：;EI(收录号：20155101691776);WOS:【SCI-EXPANDED(收录号:WOS:000372554200010)】；

基金：This work was supported by grants from the 973 Program of China (no. 2012CB721100), the 863 Program of China (no. 2012AA02A704), the 100 Talents Program of the Chinese Academy of Sciences and the National Natural Science Foundation of China (nos. 21302214, 31300040, and 31400026).

语种：英文

外文关键词：Biosynthesis; 4-Coumarate: CoA ligase; Escherichia coli; Phenyllactic acid; Rosmarinic acid analogues; Rosmarinic acid synthase

摘要：To produce rosmarinic acid analogues in the recombinant Escherichia coli BLRA1, harboring a 4-coumarate: CoA ligase from Arabidopsis thaliana (At4CL) and a rosmarinic acid synthase from Coleus blumei (CbRAS). Incubation of the recombinant E. coli strain BLRA1 with exogenously supplied phenyllactic acid (PL) and analogues as acceptor substrates, and coumaric acid and analogues as donor substrates led to production of 18 compounds, including 13 unnatural RA analogues. This work demonstrates the viability of synthesizing a broad range of rosmarinic acid analogues in E. coli, and sheds new light on the substrate specificity of CbRAS.