文献类型：期刊文献

英文题名：A novel WRKY34-bZIP3 module regulates phenolic acid and tanshinone biosynthesis in Salvia miltiorrhiza

作者：Shi, Min[1,2];Zhu, Ruiyan[1];Zhang, Yi[3];Zhang, Siwei[1];Liu, Tingyao[1];Li, Kunlun[1];Liu, Shucan[1];Wang, Leran[1];Wang, Yao[1,3];Zhou, Wei[1];Hua, Qiang[2];Kai, Guoyin[1]

机构：[1]Zhejiang Chinese Med Univ, Sch Pharmaceut Sci, Lab Med Plant Biotechnol, Hangzhou 310053, Zhejiang, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]Shanghai Normal Univ, Inst Plant Biotechnol, Sch Life Sci, Shanghai 200234, Peoples R China

年份：2022

卷号：73

起止页码：182

外文期刊名：METABOLIC ENGINEERING

收录：;EI(收录号：20223312563127);WOS:【SCI-EXPANDED(收录号:WOS:000860752400006)】；

基金：This work was supported by National Natural Science Fund of China [82073963, 31571735, 81522049] ; The Major Science and Technology Projects of Breeding New Varieties of Agriculture in Zhejiang Province [2021C02074] ; Zhejiang Provincial Ten Thousand Program for Leading Talents of Science and Technology Innovation [2018R52050] ; Zhejiang Provincial Program for the Cultivation of High-level Innovative Health Talents; Zhejiang Chinese Medical University Research Foundation [2021JKZDZC06] ; Opening Project of Zhejiang Provincial Preponderant and Characteristic Subject of Key University (Traditional Chinese Pharmacology) , Zhejiang Chinese Medical University [ZYAOXZ D2019004] .

语种：英文

外文关键词：Salvia miltiorrhiza; Hairy root; SmWRKY34; SmbZIP3; Phenolic acids; Tanshinones

摘要：Phenolic acids and tanshinones are main bioactive compounds produced in Salvia miltiorrhiza widely used in treatment of cardiovascular diseases, which could be promoted by abscisic acid elicitation. However, the regulation mechanism remained to be elucidated. An ABA-inducible IIa WRKY transcription factor (TF) named SmWRKY34 exhibiting high homology with AtWRKY40 was isolated. SmWRKY34 exhibited a negative role on phenolic acids and tanshinones by directly regulating SmRAS and SmGGPPS. Moreover, ABA-responsive bZIP TF member named SmbZIP3 expressing significantly in SmWRKY34 transcriptome was screened. SmWRKY34 showed a negative regulatory role on SmbZIP3. SmbZIP3 acted as a positive regulator in the biosynthesis of phenolic acids and tanshinones by targeting SmTAT and two tanshinone-promoting TFs SmERF128 and SmMYB9b. Taken together, we identify a new module WRKY34-bZIP3 involved in ABA signaling that manipulates phenolic acid and tanshinone accumulation, shedding new insights in metabolic engineering application in S. miltiorrhiza.