文献类型：期刊文献

中文题名：重组人铜锌SOD在脱水过程中的二级结构变化及保护剂的作用

英文题名：Investigation of Structural Changes in rhCu, Zn-SOD Induced by Dehydration and Relationship between Conformational Stability and Protectives

作者：葛宇[1];刘玲[1];高剑英[2];高俊杰[1];袁勤生[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室生物工程学院,上海200237;[2]华东理工大学化学系

年份：2003

卷号：29

期号：2

起止页码：156

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;EI(收录号：2003257514406);Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：傅立叶红外光谱;蛋白质;重组人铜锌S0D;二级结构;保护剂;脱水过程;α—螺旋结构

外文关键词：FT-IR; protein; recombinant human copper and zinc superoxide dismutase(rhCu,Zn-SOD); secondary structure

摘要：用衰减全反射(ATR)傅立叶变换红外光谱技术研究了重组人铜锌SOD(rhCu,Zn-SOD)在脱水过程中的二级结构变化及保护剂的作用。结果显示,在脱水过程中,蛋白质结构发生了重排,无论是真空干燥还是冷冻干燥,子峰峰位都发生偏移,α-螺旋结构消失,产生无规卷曲结构。糖和硼酸盐能够有效地维持酶的天然构象,其中海藻糖对冻干过程中rhCu,Zn-SOD的α-螺旋结构维持率为93%。
In this work, with attenuated total reflection fouriertransform infared spectroscopy (ATR FTIR), structural changes in rhCu,ZnSOD induced by dehydration(freezedrying and vacuumdrying)and the relationship between conformational stability and protectives were investigated. ATR FTIR is proved to be a valuable method in examining structural information of rhCu,ZnSOD in solution or dried state. The protein undergoes significant changes in the secondary structure upon freezedrying and vacuumdrying. Being dehydrated, the band positions shifted, αhelix in solution disappeared and a great amount of random coil appeared. The addition of carbohydrates and borate inhibited the protein secondary rearrangements. The efficiency can be reflected by the retain of αhelix. Trehalose appeares to be the most efficient protective. Its maintenance of αhelix upon freezedrying is 93%.