文献类型：期刊文献

英文题名：Structural characterization and immunomodulatory activity of an arabinogalactan from Jasminum sambac (L.) Aiton tea processing waste

作者：Huang, Hai[1];Yang, Xiang[1];Li, Wei[2];Han, Qifeng[1];Xu, Zhizhen[1];Xia, Wei[1];Wu, Mengqi[1,3];Zhang, Wenqing[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]Bright Dairy & Food Co Ltd, Dairy Res Inst, State Key Lab Dairy Biotechnol, Shanghai Engn Res Ctr Dairy Biotechnol, Shanghai 200436, Peoples R China;[3]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China

年份：2023

卷号：235

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：;EI(收录号：20240815592122);WOS:【SCI-EXPANDED(收录号:WOS:000996463500001)】；

基金：This work was financially supported by "Science and Technology Innovation Action Plan" for the Science and Technology Support Project in the biomedicine fields of Shanghai (22S21902800) . This research was also funded by Shanghai Engineering Research Center of Dairy Biotechnology funding, grant number 19DZ2281400.

语种：英文

外文关键词：Jasminum sambac (L; ) Aiton tea; Processing waste; Polysaccharide; Chemical structure; Immunomodulatory activity; RAW264; 7 macrophages

摘要：An arabinogalactan named JSP-1a was isolated from Jasmine tea processing waste by DEAE Sepharose FF and Sephacryl S-200 HR chromatography. Polysaccharide JSP-1a, with an average molecular weight of 87.5 kDa, was composed of galactose (59.60 %), arabinose (33.89 %), mannose (4.81 %), and rhamnose (1.70 %). JSP-1a was found to be a type II arabinogalactan comprising the main backbone of 1, 6-linked Galp residues, and the side chain containing alpha-T-Araf, alpha-1,5-Araf, beta-T-Galp, beta-1,3-Galp, and beta-1,4-Manp residues was attached to the O-3 position of beta-1,3,6-Galp residues. Evidence from bioactivity assays indicated that JSP-1a possessed potent immunomodulatory effects on RAW264.7 macrophages: treatment with JSP-1a increased phagocytosis, activated NF-kappa B p65 translocation, and promoted the production of NO, reactive oxygen species (ROS), the tumor necrosis factor (TNF)-alpha, and interleukin (IL)-6. Furthermore, inhibition of Toll-like receptor 4 caused the suppression of NO release and cytokines secretion, which indicated that TLR-4/NF-kappa B pathway might play a significant role in JSP-1a-induced macrophages' immune response. The results of this study could provide a theoretical basis of JSP1a as a safe immunostimulatory functional foods or a treatment for immunological diseases.