文献类型：期刊文献

英文题名：A Time-Resolved Single-Molecular Train Based on Aerolysin Nanopore

作者：Ying, Yi-Lun[1,2];Li, Zi-Yuan[1,2];Hu, Zheng-Li[1,2];Zhang, Junji[1,2];Meng, Fu-Na[1,2];Cao, Chan[1,2];Long, Yi-Tao[1,2];Tian, He[1,2]

机构：[1]East China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China

年份：2018

卷号：4

期号：8

起止页码：1893

外文期刊名：CHEM

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000441194300013)】；

基金：This research was supported by the National Natural Science Foundation of China (21421004, 21327807, and 21505043) and the Fundamental Research Funds for the Central Universities (222201718001, 222201717003, and 222201714012).

语种：英文

摘要：An aerolysin nanopore interface was introduced as a molecular machine to electrically read out the real-time photo-controlled motion of an azobenzene-geared DNA train with high spatial and temporal resolution. Under alternating UV and visible irradiation, each DNA train performed two regulated speeds of 1.9 and 6.3 bases/s corresponding to trans and cis states, respectively, with readily identified current signals. Each train type fell into the ultra-narrow current population with a full-width half maximum of 0.2-0.4 pA. The combination of a model molecular machine system and powerful aerolysin interface enabled the motions of every artificial molecular machine to be followed in real time.