文献类型：期刊文献

英文题名：Synthesis of magnetic zwitterionic-hydrophilic material for the selective enrichment of N-linked glycopeptides

作者：Zhao, Yiman[1];Chen, Yajing[1];Xiong, Zhichao[2];Sun, Xudong[1];Zhang, Quanqing[3];Gan, Yangyang[1];Zhang, Lingyi[1];Zhang, Weibing[1]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Shanghai Inst Ceram, State Key Lab High Performance Ceram & Superfine, Shanghai 200050, Peoples R China;[3]Fudan Univ, Shanghai 200433, Peoples R China

年份：2017

卷号：1482

起止页码：23

外文期刊名：JOURNAL OF CHROMATOGRAPHY A

收录：;EI(收录号：20170203238376);WOS:【SCI-EXPANDED(收录号:WOS:000392681500003)】；

基金：We gratefully acknowledge the support of the National Natural Science Foundation of China (No.21475044), the National Key Scientific Instrument and Equipment Development Project (2012YQ120044), the State Key Program of National Science of China (21235005), the National Key Technology R&D Program(2015BAK44B00), and foundation of Shanghai Research Institute of Criminal Science and Technology.

语种：英文

外文关键词：Magnetic nanoparticles; Glycopeptides; Zwitterionic-hydrophilic interaction liquid chromatography; Enrichment; Mass spectrum

摘要：In consideration of the close connection between glycopeptides and human diseases, the efficient method to separate and enrich glycopeptides from complex biological samples is urgently required. In the work, we developed a magnetic zwitterionic-hydrophilic material for highly effective separation and analysis of glycopeptides from complex samples. The Fe3O4 particles were covered with a thick layer of polymer by one-step reflux-precipitation polymerization (RPP), subsequently decorated by Au nanoparticles (Au NPs) through in situ reduction and finally modified with zwitterionic groups. The abundant zwitterionic sites facilitate the selective enrichment of glycopeptides. Besides, the prepared Fe3O4@PGMA@Au-Lcys showed high detection sensitivity (5 fmol IgG digest), approving enrichment capacity (75 mg g(-1)), satisfactory enrichment recovery (89.8%), and great performance in the analysis and profiling of low abundance N-linked glycopeptides. Furthermore, the prepared material was employed in the enrichment of glycopeptides in intricate biological samples, and 774 unique N-glycosylation sites from 411 N-glycosylated proteins were reliably identified in three replicate analyses of a 75 mu g protein sample extracted from mouse liver, suggesting wide application prospect in glycoproteomics. (C) 2016 Elsevier B.V. All rights reserved.