文献类型：期刊文献

英文题名：Enhancement of erythromycin A production with feeding available nitrogen sources in erythromycin biosynthesis phase

作者：Zou, Xiang[1,2];Hang, Hai-feng[1];Chu, Ju[1];Zhuang, Ying-ping[1];Zhang, Si-liang[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Southwest Univ, Coll Pharmaceut Sci, Chongqing 400715, Peoples R China

年份：2009

卷号：100

期号：13

起止页码：3358

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20091512020682);WOS:【SCI-EXPANDED(收录号:WOS:000265810900027)】；

基金：This work was financially supported by a Grant from the National High Technology research and Development Program of China (863 Program), No.2006AA020304, and the Major State Basic Research Development Program of China (973 Program), No. 2007CB714303.

语种：英文

外文关键词：Available nitrogen sources; Biosynthesis phase; Erythromycin A; Industrial scale; Scale up

摘要：Effects of feeding different available nitrogen sources from 80 h in erythromycin biosynthesis phase on the erythromycin A (Er-A) production were investigated in 50 I fermenter. Feeding corn steep liquor and yeast extract, the Er-A production was enhanced, while the biotransformation from erythromycin C (Er-C) to Er-A had no increase. When ammonium sulphate was fed at high feeding rate, the maximal Er-A production and ratio of Er-A to Er-C were 7953 U/ml and 98.18:1 at 184 h. respectively, which were higher than that of the control (6742 U/ml and 5.47:1). The feeding ammonium sulphate process was successfully scaled up from 501 to 25 m(3) fermenter. The maximal Er-A production reached 7938 U/ml at 203 h, which was enhanced by 22.1% compared with the control (6501 U/ml at 192 h). The ratio of Er-A to Er-C was 24.05:1, which was higher than that of the control (4.77:1). (C) 2009 Elsevier Ltd. All rights reserved.