文献类型：期刊文献

英文题名：Burkholderia jiangsuensis sp nov., a methyl parathion degrading bacterium, isolated from methyl parathion contaminated soil

作者：Liu, Xu-Yun[1];Li, Chun-Xiu[1];Luo, Xiao-Jing[1];Lai, Qi-Liang[2,3,4];Xu, Jian-He[1]

机构：[1]E China Univ Sci & Technol, Lab Biocatalysis & Synthet Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]State Key Lab Breeding Base Marine Genet Resource, Xiamen 361005, Peoples R China;[3]SOA, Inst Oceanog 3, Key Lab Marine Genet Resources, Xiamen 361005, Peoples R China;[4]Key Lab Marine Genet Resources Fujian Prov, Xiamen 361005, Peoples R China

年份：2014

卷号：64

起止页码：3247

外文期刊名：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000344912300048)】；

基金：This work was supported financially by the Ministry of Science and Technology (2012AA022206C and 2011CB710800). The authors are grateful to Professor Qing Hong at Nanjing Agricultural University for kindly providing type strains.

语种：英文

摘要：A methyl parathion (MP) degrading bacterial strain, designated MP-1(T), was isolated from a waste land where pesticides were formerly manufactured in Jiangsu province, China. Polyphasic taxonomic studies showed that MP-1(T) is a Gram-stain-negative, non-spore-forming, rod-shaped and motile bacterium. The bacterium could grow at salinities of 0-1% (w/v) and temperatures of 15-40 degrees C. Strain MP-1(T) could reduce nitrate to nitrite, utilize D-glucose and L-arabinose, but not produce indole, or hydrolyse gelatin. Phylogenetic analysis based on 16S rRNA gene sequences demonstrated that MP-1(T) belongs to the genus Burkholderia, showing highest sequence similarity to Burkholderia grimmiae DSM 25160(T) (98.5%), and similar strains including Burkholderia zhejiangensis OP-1(T) (98.2%), Burkholderia choica LMG 22940(T) (97.5 %), Burkholderia glathei DSM 50014(T) (97.4%), Burkholderia terrestris LMG 22937(T) (97.2%) and Burkholderia telluris LMG 22936(T) (97.0%). In addition, the gyrB and recA gene segments of strain MP-1(T) exhibited less than 89.0% and 95.1% similarities with the most highly-related type strains indicated above. The G+C content of strain MP-1(T) was 62.6 mol%. The major isoprenoid quinone was ubiquinone Q-8. The predominant polar lipids comprised phosphatidyl ethanolamine, phosphatidyl glycerol, aminolipid and phospholipid. The principal fatty acids in strain MP-1(T) were C-18:1 omega 7c/C-18:1 omega 6c (23.3 %), C-16:0 (16.8 %), cyclo-C-17:0 (15.0 %), C-16:1 omega 7c/C-16:1 omega 6 (8.5 %), cyclo-C-19:0 omega 8c (8.1 %), C-16:1 iso I/C-14:0 3-OH (5.7%), C-16:0 3-OH (5.6%) and C(16:0)2-OH (5.1 %). The DNA-DNA relatedness values between strain MP-1(T) and the three type strains (B. grimmiae DSM 25160(T), B. zhejiangensis OP-1(T) and B. glathei DSM 50014(T)) ranged from 24.6% to 37.4%. In accordance with phenotypic and genotypic characteristics, strain MP-1(T) represents a novel species of the genus Burkholderia, for which the name Burkholderia jiangsuensis sp. nov. is proposed, the type strain is MP-1(T) (LMG 27927(T)=MCCC 1K00250(T)).