文献类型：期刊文献

中文题名：鸡胚细胞的培养

英文题名：Culture of Chicken Embryo Cells

作者：张立[1];张元兴[1];华平[1];俞俊棠[1]

机构：[1]华东理工大学生化工程研究所

年份：1994

卷号：20

期号：3

起止页码：331

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;国家哲学社会科学学术期刊数据库;EI(收录号：1994121437932);Scopus;CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：细胞生长;微载体;鸡胚;胶原;培养基;DMEM;初步探讨;积累规律;养鸡;生长规律

外文关键词：chicken embryo cell;cell culture; culture medium;microcarrier;glucosc;lactic acid;ammonia

摘要：研究了鸡胚细胞的生长规律，葡萄糖的利用和乳酸、氨等代谢产物的积累规律，比较了培养基对细胞生长的促进作用，筛选了合适的微载体，初步探讨了用微载体培养鸡胚细胞的方法。实验结果表明，鸡胚细胞在ＤＭＥＭ培养基中生长良好，最适合的微载体为胶原型微载体，如ＧＴ－２，Ｃｙｔｏｄｅｘ－３。
The growth of Chicken embryo cells,the utilization of glucose and the accumulation of metabolic products such as lactic acid and ammonia were investgated.The progressive effects of media to the chicken embryo cell growth were compared.The suitable microcarriels were chosen.And the methods of chicken embryo cell culture by microcarricrs were given. It was found that chicken embryo cells grow well in DMEM mcdium and the optimum micro-carrier is collagen-type microcarrier,such as GT-2, Cytodex-3.