文献类型：期刊文献

英文题名：Simple, ultrasensitive detection of superoxide anion radical mutations in melanoma mice with SERS microneedles

作者：He, Miao[1];Jin, Lili[1];Wang, Feng[2];Wang, Xin[3];You, Yanli[3];He, Hongyan[1]

机构：[1]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Engn Res Ctr Biomed Mat, Minist Educ, Shanghai 200237, Peoples R China;[2]Shanghai Normal Univ, Shanghai Frontiers Sci Ctr Biomimet Catalysis, Joint Int Res Lab Resource Chem,Minist Educ, Shanghai Key Lab Rare Earth Funct Mat,Educ Minist, Shanghai 200234, Peoples R China;[3]Second Mil Med Univ, Dept Tradit Chinese Med, Shanghai 200433, Peoples R China

年份：2024

卷号：316

外文期刊名：SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY

收录：;EI(收录号：20241715975470);WOS:【SCI-EXPANDED(收录号:WOS:001234158400001)】；

语种：英文

外文关键词：SERS; O2 & sdot;-detection; Melanoma; Microneedle

摘要：Elevated levels of superoxide anion radicals (O 2 & sdot;- ) have been implicated in the pathogenesis of a variety of diseases, such as cancer, inflammatory diseases and autoimmune diseases. To determine the O 2 & sdot;- concentration for assisting disease detection, a method based on surface-enhanced Raman scattering (SERS) combined with transparent polymer microneedles has been developed. Photocrosslinked NOA61 is used to prepare microneedles with sulfhydryl group, which can contribute to anchor gold nanoparticles (Au NPs) functionalized by p-mercaptobenzoic acid (PATP). This work successfully constructed SERS microneedles for in situ detection. A REDOX reaction occurred between PATP and O 2 & sdot;- , resulting in the formation of dimethylaminoborane (DMAB) and a subsequent change in Raman signal. Based on the quantitative relationship between the change of peak area ratio at 1042 cm -1 and 1077 cm -1 and the concentration change of O 2 & sdot;- , a standard curve with a linear range of 0 -480 ng/mL was constructed. The SERS microneedles were effectively employed to track melanoma progression in mice, establishing a fundamental correlation between O2 & sdot;- concentration and melanoma stage, as confirmed by ELISA. The benefits of this approach, including convenience, in situ applicability, and low cost, are anticipated to offer novel insights for non-invasive in situ detection, potentially enhancing disease monitoring and diagnosis.