文献类型：期刊文献

英文题名：POSS-enhanced thermosensitive hybrid hydrogels for cell adhesion and detachment

作者：Tong, Yudong[1];Zhang, Yuanhao[2];Liu, Yangyang[1];Cai, Haibo[1];Zhang, Weian[2];Tan, Wen-Song[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2018

卷号：8

期号：25

起止页码：13813

外文期刊名：RSC ADVANCES

收录：;EI(收录号：20181705050831);WOS:【SCI-EXPANDED(收录号:WOS:000430538300025)】；

基金：This work was financially supported by the National Natural Science Foundation of China (grant number 21574039), the Science and Technology Commission of Shanghai Municipality (grant number 15JC1401402), and the Open Funding Project of the State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：Irradiation - Cell culture - Crosslinking - Acrylic monomers - Amides - Cell proliferation - Cell adhesion - Cell engineering - Stem cells - Biomechanics

摘要：Thermosensitive poly(N-isopropylacrylamide) (PNIPAM)-based substrates have presented great promise in cell sheet engineering. However, non-functionalized PNIPAM cannot be well applied for cell cultivation, due to the low cell adhesion. Herein, to enhance PNIPAM-based substrates and to promote cell proliferation and detachment, a polyhedral oligomeric silsesquioxane (POSS) nanoscale inorganic enhanced agent has been introduced into PNIPAM matrices to construct POSS-containing hybrid hydrogels. The hydrogels were facilely prepared using POSS as a cross-linker via one-pot crosslinking reaction under UV irradiation. The swelling behavior, thermal stability and the mechanical properties of POSS-PNIPAM hybrid hydrogels have been evaluated and they are all dependent on the content of POSS. The in vitro experiment confirms that human amniotic mesenchymal stem cells (hAMSCs) exhibit clearly enhanced adhesion and proliferation on the substrates of POSS-PNIPAM hybrid hydrogels in comparison to the pure PNIPAM hydrogel without POSS. Based on the thermal-responsiveness of PNIPAM, the proliferated cells are easily released without damage from the surface of hybrid hydrogels. Therefore, POSS-enhanced PNIPAM hybrid hydrogels provide a unique approach for harvesting anchorage dependent stem cells.