文献类型：期刊文献

英文题名：Adsorptive bioconversion of ethylene glycol to glycolic acid by Gluconobacter oxydans DSM 2003

作者：Wei, Guodong[1];Yang, Xuepeng[2];Zhou, Wenyu[1];Lin, Jinping[1];Wei, Dongzhi[1]

机构：[1]E China Univ Sci & Technol, New World Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Zhengzhou Univ Light Ind, Coll Food & Biol Engn, Zhengzhou 450002, Peoples R China

年份：2009

卷号：47

期号：1-3

起止页码：127

外文期刊名：BIOCHEMICAL ENGINEERING JOURNAL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000274349600019)】；

基金：This work was supported by the National Basic Research Program (973 program, No. 2009CB724703) and the program Shanghai Subject Chief Scientist (No. 06XD14006).

语种：英文

外文关键词：Gluconobacter oxydans; Glycolic acid; Ethylene glycol; Product inhibition; Integrated bioprocess; In situ product removal

摘要：An integrated bioprocess for the production of glycolic acid from ethylene glycol with Gluconobacter oxydans DSM 2003 and in situ product removal were investigated. A slight substrate inhibition was observed as substrate concentration was above 20 g/l and the product inhibition was much stronger. Bioconversion of glycolic acid is an end-product-inhibited reaction. In order to increase the productivity of glycolic acid and reduce the end-product inhibition of bioconversion, an adsorptive bioconversion for glycolic acid production from ethylene glycol catalyzed by resting cells of G. oxydans DSM 2003. was developed by using anion exchange resin D315 as the adsorbent for selective removal of glycolic acid from the reaction mixture. This approach allowed the yield of glycolic acid to be increased to 93.2 g/l, compared to 74.5 g/l obtained from a conventional fed-batch mode. (C) 2009 Elsevier B.V. All rights reserved.