文献类型：期刊文献

英文题名：Electrochemical detection of DNA hybridization by using a zirconia modified renewable carbon paste electrode

作者：Zuo, Shao-Hua[1,2];Zhang, Ling-Fan[1,2];Yuan, Hui-Hui[3];Lan, Min-Bo[1,2,3];Lawrance, Geoffrey A.[4];Wei, Gang[5]

机构：[1]E China Univ Sci & Technol, Res Ctr Anal & Test, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Inst Adv Mat, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, Chinese Educ Minist, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China;[4]Univ Newcastle, Sch Environm & Life Sci, Discipline Chem, Callaghan, NSW 2308, Australia;[5]CSIRO Ind Phys, Lindfield, NSW 2070, Australia

年份：2009

卷号：74

期号：2

起止页码：223

外文期刊名：BIOELECTROCHEMISTRY

收录：;EI(收录号：20090511877787);WOS:【SCI-EXPANDED(收录号:WOS:000263529200001)】；

基金：The support of the Science and Technology Commission of Shanghai Municipality (STCSM; No 065407061) is gratefully acknowledged.

语种：英文

外文关键词：Carbon paste electrode; DNA hybridization; Zirconia; Methylene blue; Electrochemical DNA sensor

摘要：A simple, polishable and renewable DNA biosensor was fabricated based on a zirconia modified carbon paste electrode. Zirconia was mixed with graphite powder and paraffin wax to produce the paste for the electrode. and response-optimized at 56% graphite powder, 19% ZrO2 and 25% paraffin wax. An oligonucleotide probe with a terminal 5'-phosphate group was attached to the Surface of the electrode via the strong affinity of zirconia for phosphate groups. DNA immobilization and hybridization were characterized by cyclic voltammetry and differential Pulse voltammetry, using methylene blue as indicator. Examination of changes in response with complementary or non-complementary DNA sequences showed that the developed biosensor had a high selectivity and sensitivity towards hybridization detection (<= 2 x 10(-10) M complementary DNA detectable). The surface of the biosensor can be renewed quickly and reproducibly (signal RSD +/- 4.6% for five successive renewals) by a simple polishing step. (C) 2008 Elsevier B.V. All rights reserved.