文献类型：期刊文献

英文题名：In vitro allosteric transcription factor-based biosensing

作者：Li, Shanshan[1];Li, Zilong[2];Tan, Gao-Yi[3,4];Xin, Zhenguo[2,5];Wang, Weishan[2,5]

机构：[1]Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China;[2]Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China;[3]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[4]East China Univ Sci & Technol, Sch Biotechnol, Shanghai 200237, Peoples R China;[5]Univ Chinese Acad Sci, Beijing 100049, Peoples R China

年份：2023

卷号：41

期号：8

起止页码：1080

外文期刊名：TRENDS IN BIOTECHNOLOGY

收录：;EI(收录号：20231313807097);WOS:【SCI-EXPANDED(收录号:WOS:001147654200001)】；

基金：This work was supported by the National Key Research and Development Program of China (2021YFC2100600 and 2020YFA0907800) , the National Natural Science Foundation of China (32172502, 32170095, 31972348, and 31922002) , and the Youth Innovation Promotion Association CAS (Y202027) to W.W.

语种：英文

外文关键词：Analytic equipment - Molecules - Transcription - Transcription factors

摘要：A biosensor is an analytical device that converts a biological response into a measurable output signal. Bacterial allosteric transcription factors (aTFs) have been utilized as a novel class of recognition elements for in vitro biosensing, which circumvents the limitations of aTF-based whole-cell biosensors (WCBs) and helps to meet the increasing requirement of small-molecule biosensors for diverse applications. In this review, we summarize the recent advances related to the configuration of aTF-based biosensors in vitro. Particularly, we evaluate the advantages of aTFs for in vitro biosensing and highlight their great potential for the establishment of robust and easy-to-implement biosensing strategies. We argue that key technical innovations and generalizable workflows will enhance the pipeline for facile construction of diverse aTF-based small-molecule biosensors.