文献类型：期刊文献

英文题名：Engineering a favourable osteogenic microenvironment by heparin mediated hybrid coating assembly and rhBMP-2 loading

作者：Tan, Haoqi[1,2];Wang, Honglei[1,2];Chai, Yanjun[1,2];Yu, Yuanman[1,2];Hong, Hua[1,2];Yang, Fei[3];Qu, Xue[1,2];Liu, Changsheng[1,2]

机构：[1]East China Univ Sci & Technol, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]Chinese Acad Sci, Inst Chem, State Key Lab Polymer Phys & Chem, Beijing 100190, Peoples R China

年份：2017

卷号：7

期号：19

起止页码：11439

外文期刊名：RSC ADVANCES

收录：;EI(收录号：20170803378822);WOS:【SCI-EXPANDED(收录号:WOS:000395867900029)】；

基金：This work was supported by National Natural Science Foundation of China (51573047, E0213), Innovation Program of Shanghai Municipal Education Commission (14ZZ060), Shanghai Science and Technology Development Funds (14QA1401000), and the 111 project (B14018).

语种：英文

外文关键词：Bone - Cell adhesion - Recombinant proteins - Hydroxyapatite - Nanoparticles - Biocompatibility - Cell engineering - Coatings - Tissue engineering

摘要：With the development of bone tissue engineering, it is a great challenge to fabricate a bioactive surface which can provide a favourable microenvironment for cell adhesion, migration and osteo-differentiation. Coating the as-prepared substrates with bioactive components is an effective method to induce osteogenesis. In this study, we designed a multi-layered hybrid coating containing hydroxyapatite nanoparticles, heparin and chitosan. Heparin is used to mediate the electrostatic assembly of hydroxylapatite nanoparticles with chitosan, as well as the subsequent recombinant human bone morphogenetic protein-2 (rhBMP-2) loading. Several independent pieces of evidence were provided to indicate the successful fabrication of the hybrid coating. Sustained release of rhBMP-2 by this coating was also achieved. The in vitro biological studies reveal that the hybrid coating system has excellent biocompatibility and can improve the osteo-differentiation especially for rhBMP-2 present on the coating surface. The results demonstrate that the proposed rhBMP-2 loaded hybrid coating can provide a favourable osteogenic microenvironment and has the potential to improve in situ bone repair due to its superior osteoconductivity as well as osteoinductivity.