文献类型：期刊文献

中文题名：酿酒酵母重组人α2a干扰素补料分批培养

英文题名：Production of Recombinant Human Interferon α2a by Saccharomyces cerevisiae in Fed-batch Culture

作者：储炬[1];胡千德[1];郭元昕[1];叶勤[1];林志伟[2];徐伦[2]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237;[2]上海万兴生物制品有限公司,上海201206

年份：2001

卷号：27

期号：4

起止页码：349

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：酿酒酵母;基因工程菌;α2a型干扰素;补料分批培养;腺嘌呤;抗病毒药物;抗肿瘤多肽药物

外文关键词：Saccharomyces cerevisiae; cultivation of recombinant yeast; IFN α2a, fed batch cultivation; adenine

摘要：在 2 .6L全自动发酵罐中对人 α2 a干扰素重组酵母的补料分批培养过程作了初步研究。结果表明 ,培养过程中待葡萄糖耗尽时流加葡萄糖 ,并维持低糖浓度 ;在培养 1 0 h～ 2 0 h时 ,恒速流加 2 0 mg/L的腺嘌呤 ,能较大幅度提高表达水平 ,生物活性从原工艺的 3.1× 1 0 9IU/L提高至1 .3× 1 0 10 IU/L。表达期 p H值对生产水平有很大影响 ,宜控制在 5.0～ 5.
Production of recombinant human interferon α2a by Saccharomyces cerevisiae in fed batch culture was investigated systematically in 2.6 L automatic jar fermenter. During the fermentation, suitable amount of glucose was added to keep the glucose concentration at low level, when glucose was exhausted; 20mg/L of adenine was supplemented at even speed during 10h～20h of fermentation. As a consequence, the expression level of human interferon α2a has increased by 4 times to 1.3×10 10 IU/L. In expression phase, pH value has an important impact on expression level, which should be controlled within 5.0～5.3.