文献类型：期刊文献

英文题名：Covalent Functionalization of Graphene Oxide with Biocompatible Poly(ethylene glycol) for Delivery of Paclitaxel

作者：Xu, Zhiyuan[1,2];Wang, Song[2];Li, Yongjun[1];Wang, Mingwei[3];Shi, Ping[2];Huang, Xiaoyu[1]

机构：[1]Chinese Acad Sci, Shanghai Inst Organ Chem, Key Lab Synthet & Self Assembly Chem Organ Funct, Shanghai 200032, Peoples R China;[2]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]Fudan Univ, Dept Nucl Med, Shanghai Canc Ctr, Shanghai 200032, Peoples R China

年份：2014

卷号：6

期号：19

起止页码：17268

外文期刊名：ACS APPLIED MATERIALS & INTERFACES

收录：;EI(收录号：20144300115991);WOS:【SCI-EXPANDED(收录号:WOS:000343018200095)】；

基金：The authors are thankful for the financial support of the National Natural Science Foundation of China (21204098, 31100549, and 11275050), Shanghai Scientific and Technological Innovation Project (11nm0501100, 11ZR1445900, 14520720100, and 14520720700), State Key Laboratory of Bioreactor Engineering (2060204), and Fundamental Research Funds for the Central Universities (222201313010). We thank Prof. Guowei Wang (Fudan Univeristy) for assistance in synthesizing 6-armed PEG.

语种：英文

外文关键词：graphene oxide; PEG; paclitaxel; A549; MCF-7

摘要：Graphene oxide (GO), a novel 2D nanomaterial prepared by the oxidation of natural graphite, has been paid much attention in the area of drug delivery due to good biocompatibility and low toxicity. In the present work, 6-armed poly(ethylene glycol) was covalently introduced into the surface of GO sheets via a facile amidation process under mild conditions, making the modified GO, GO-PEG (PEG: 65 wt %, size: 50-200 nm), stable and biocompatible in physiological solution. This nanosized GO-PEG was found to be nontoxic to human lung cancer A549 and human breast cancer MCF-7 cells via cell viability assay. Furthermore, paclitaxel (PTX), a widely used cancer chemotherapy drug, was conjugated onto GO-PEG via pi-pi stacking and hydrophobic interactions to afford a nanocomplex of GO-PEG/PTX with a relatively high loading capacity for PTX (11.2 wt %). This complex could quickly enter into A549 and MCF-7 cells evidenced by inverted fluorescence microscopy using Fluorescein isothiocyanate as a probe, and it also showed remarkably high cytotoxicity to A549 and MCF-7 cells in a broad range of concentration of PTX and time compared to free PTX. This kind of nanoscale drug delivery system on the basis of PEGylated GO may find potential application in biomedicine.